Assessment of IL-8 Levels in Saliva of Healthy and Chronic Periodontitis Individuals

Inflammation of the gums and other tissues supporting the teeth, as well as gradual loss of attachment and bone, are the results of chronic periodontitis, an infectious illness. During inflammation, a group of low molecular weight proteins called cytokines facilitate a complex interaction between in...

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Bibliographic Details
Published in:Journal of pharmacy & bioallied science 2024-02, Vol.16 (Suppl 1), p.S825-S827
Main Authors: Suryavanshi, Vinaykumar G, Tale, Ravi K, Aasole, Ankush G, Barge, Arti K, Sanikop, Sheetal, Gopashetti, Pallavi
Format: Article
Language:English
Subjects:
chronic periodontitis
Connective tissues
elisa
Enzyme-linked immunosorbent assay
Gum disease
il-8
Inflammation
interleukin
Interleukin 8
Interleukins
Leukocytes (neutrophilic)
Molecular weight
Original
Periodontitis
Saliva
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Summary:Inflammation of the gums and other tissues supporting the teeth, as well as gradual loss of attachment and bone, are the results of chronic periodontitis, an infectious illness. During inflammation, a group of low molecular weight proteins called cytokines facilitate a complex interaction between inflammatory cells (such neutrophils) and other cellular components in connective tissue. The cytokine interleukin-8 (IL-8) is a potent neutrophil chemoattractant. Therefore, it is possible that IL-8 is crucial to the development of periodontitis's pathology. 1) To estimate concentration of IL-8 levels in healthy individuals and chronic periodontitis individuals. 2) To compare IL-8 levels in healthy and chronic periodontitis individuals. Participants in this research will be recruited from among those who visit the outpatient department (OPD) at the NGH Institute of Dental Sciences and Research Centre, Belagavi, run by the Maratha Mandal. Subjects with no clinical attachment loss (CAL) and a probing depth of 3.0 mm are considered to be periodontally healthy. Those in Group 2 (chronic periodontitis) have a chronic form of the disease, as shown by a probing pocket depth (PPD) of less than 5 mm and CAL of less than 2 mm. Unstimulated saliva sample will be collected in a 5 mL wide-mouthed sterile container by spitting method. Samples collected will be centrifuged. The supernatant is collected and stored at -80°C and then assayed for IL-8 concentration by using the standardized IL-8 ELISA kit.
ISSN:0976-4879
0975-7406
DOI:10.4103/jpbs.jpbs_1041_23