Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment

Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, derived EVs were tested as carriers for the widely used chemotherapeutic...

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Published in:Drug delivery 2025-12, Vol.32 (1), p.2439272
Main Authors: Steć, Aleksandra, Targońska, Monika, Jaikishan, Shishir, Chen, Rui, Mucha, Piotr, Czyrski, Grzegorz S, Jasiecki, Jacek, Płoska, Agata, Heinz, Andrea, Wiedmer, Susanne K, Kalinowski, Leszek, Waleron, Krzysztof, Wielgomas, Bartosz, Dziomba, Szymon
Format: Article
Language:English
Subjects:
Antibiotics, Antineoplastic - administration & dosage
Antibiotics, Antineoplastic - pharmacokinetics
Antibiotics, Antineoplastic - pharmacology
Capillary electrophoresis
Cell Survival - drug effects
Citrus - chemistry
Doxorubicin - administration & dosage
Doxorubicin - pharmacokinetics
Doxorubicin - pharmacology
Drug Carriers - chemistry
drug incorporation
exosome-like vesicles
extracellular vesicles
Extracellular Vesicles - chemistry
Extracellular Vesicles - metabolism
HEK293 Cells
HeLa Cells
Humans
nanocarriers
Nanoparticles - chemistry
nanoplasmonic sensing
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Summary:Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.
ISSN:1071-7544
1521-0464
1521-0464
DOI:10.1080/10717544.2024.2439272