Characterization of a New and Efficient Polyvalent Phage Infecting E. coli O157:H7, Salmonella spp., and Shigella sonnei

Ongoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density ce...

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Bibliographic Details
Published in:Microorganisms (Basel) 2021-10, Vol.9 (10), p.2105
Main Authors: Kim, Su-Hyeon, Adeyemi, Damilare Emmanuel, Park, Mi-Kyung
Format: Article
Language:English
Subjects:
Abattoirs
Allergenicity
Antibiotic resistance
Antibiotics
Bacteria
Biological control
Burst size
Cellulose acetate
Centrifugation
Cesium
E coli
effectiveness
Efficiency
Escherichia coli O157:H7
Foodborne diseases
Genes
Genomes
Genomic analysis
Host range
Laboratories
Morphology
Pathogens
Phages
Polyethylene glycol
polyvalent phage
Public health
Salmonella
Sewage
Shigella sonnei
Virulence
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Summary:Ongoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density centrifugation. Host range and efficiency of plating analyses revealed that KFS-EC3 is polyvalent and can efficiently infect E. coli O157:H7, Salmonella spp., and Shigella sonnei. KFS-EC3 had a latent time of 20 min and burst size of ~71 phages/infected cell. KFS-EC3 was stable and infectious following storage at a pH range of 3 to 11 and a temperature range of −70 °C to 60 °C. KFS-EC3 could inhibit E. coli O157:H7 growth by 2 logs up to 52 h even at the lowest MOI of 0.001. Genomic analysis of KFS-EC3 revealed that it consisted of 167,440 bp and 273 ORFs identified as functional genes, without any genes associated with antibiotic resistance, virulence, allergenicity, and lysogenicity. This phage was finally classified into the Tequatrovirus genus of the Myoviridae family. In conclusion, KFS-EC3 could simultaneously infect E. coli O157:H7, S. sonnei, and Salmonella spp. with the lowest MOI values over long periods, suggesting its suitability for simultaneous pathogen control in foods.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9102105