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Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test
Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promas...
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| Published in: | Parasites & vectors 2024-09, Vol.17 (1), p.402-402, Article 402 |
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| description | Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.
Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.
The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P |
| doi_str_mv | 10.1186/s13071-024-06486-0 |
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Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.
The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO: 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.
AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/s13071-024-06486-0</identifier><identifier>PMID: 39334233</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adolescent ; Adult ; Antigen-antibody reactions ; antigens ; Antigens, Protozoan - immunology ; Axenic amastigote antigen ; Axenic promastigote antigen ; Brazil ; cell-mediated immunity ; cutaneous leishmaniasis ; delayed hypersensitivity ; Diagnosis ; Evaluation ; Female ; Humans ; Laboratory diagnosis ; Leishmania ; Leishmania (V.) braziliensis ; Leishmania (V.) lainsoni ; Leishmania - immunology ; Leishmaniasis ; Leishmaniasis, Cutaneous - diagnosis ; Leishmaniasis, Cutaneous - immunology ; Leishmaniasis, Cutaneous - parasitology ; Male ; Methods ; Middle Aged ; Montenegro skin test ; parasites ; Physiological aspects ; Skin tests ; Skin Tests - methods ; t-test ; Young Adult</subject><ispartof>Parasites & vectors, 2024-09, Vol.17 (1), p.402-402, Article 402</ispartof><rights>2024. The Author(s).</rights><rights>COPYRIGHT 2024 BioMed Central Ltd.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c484t-b642b5f9b1ba07630c50513c643e2b7a81a794d11b8d32d8b7a883366d23beba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438107/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438107/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,37011,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39334233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Melo, Leonardo Viana</creatorcontrib><creatorcontrib>Vasconcelos Dos Santos, Thiago</creatorcontrib><creatorcontrib>Ramos, Patrícia Karla</creatorcontrib><creatorcontrib>Lima, Luciana Vieira</creatorcontrib><creatorcontrib>Campos, Marliane Batista</creatorcontrib><creatorcontrib>Silveira, Fernando Tobias</creatorcontrib><title>Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.
Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.
The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO: 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.
AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antigen-antibody reactions</subject><subject>antigens</subject><subject>Antigens, Protozoan - immunology</subject><subject>Axenic amastigote antigen</subject><subject>Axenic promastigote antigen</subject><subject>Brazil</subject><subject>cell-mediated immunity</subject><subject>cutaneous leishmaniasis</subject><subject>delayed hypersensitivity</subject><subject>Diagnosis</subject><subject>Evaluation</subject><subject>Female</subject><subject>Humans</subject><subject>Laboratory diagnosis</subject><subject>Leishmania</subject><subject>Leishmania (V.) braziliensis</subject><subject>Leishmania (V.) lainsoni</subject><subject>Leishmania - immunology</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis, Cutaneous - diagnosis</subject><subject>Leishmaniasis, Cutaneous - immunology</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Male</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Montenegro skin test</subject><subject>parasites</subject><subject>Physiological aspects</subject><subject>Skin tests</subject><subject>Skin Tests - methods</subject><subject>t-test</subject><subject>Young Adult</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqNks1u1DAUhSMEoqXwAiyQJTbtIsWOHcdZoVHFz0iDkPjbWteOk7ok9tT2jFpeglfGmSlVR2KBvMjV9XfOVa5PUbwk-JwQwd9EQnFDSlyxEnMmeIkfFcekqXlJKa4fP6iPimcxXmHMcVvzp8URbSllFaXHxe-FS3YwzmoUDOhktzbdIt-jlbHxcgJnAZ3-sOBycYZGsC56ZxHc7CQwQcxynwxaB781HUoeKYMAxY1NoMZcjskEB9nYoN4H5NfJTvaXdQP65F0yzgzBo_jTOpRMTM-LJz2M0by4-54U39-_-3bxsVx9_rC8WKxKzQRLpeKsUnXfKqIAN5xiXeOaUM0ZNZVqQBBoWtYRokRHq07MLUEp511FlVFAT4rl3rfzcCXXwU4QbqUHK3cNHwYJIVk9GglYZ1nX1HXbM9J2wnRa0R4IJoIZPHu93XutN2rKl8alAOOB6eGNs5dy8FtJCKOC4CY7nN45BH-9yWuQk43ajCM44zdRUlJnUHDG_gMluMW84VVGX-_RAfJvWNf7PF3PuFzksQwz3s6zz_9B5dOZyWrvTG9z_0BwdiDQ8zPepAE2Mcrl1y-HbLVndfAxBtPfb4VgOYdY7kMsc4jlLsQSZ9Grh_u8l_xNLf0DeKLtkA</recordid><startdate>20240927</startdate><enddate>20240927</enddate><creator>de Melo, Leonardo Viana</creator><creator>Vasconcelos Dos Santos, Thiago</creator><creator>Ramos, Patrícia Karla</creator><creator>Lima, Luciana Vieira</creator><creator>Campos, Marliane Batista</creator><creator>Silveira, Fernando Tobias</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20240927</creationdate><title>Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test</title><author>de Melo, Leonardo Viana ; Vasconcelos Dos Santos, Thiago ; Ramos, Patrícia Karla ; Lima, Luciana Vieira ; Campos, Marliane Batista ; Silveira, Fernando Tobias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-b642b5f9b1ba07630c50513c643e2b7a81a794d11b8d32d8b7a883366d23beba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Antigen-antibody reactions</topic><topic>antigens</topic><topic>Antigens, Protozoan - immunology</topic><topic>Axenic amastigote antigen</topic><topic>Axenic promastigote antigen</topic><topic>Brazil</topic><topic>cell-mediated immunity</topic><topic>cutaneous leishmaniasis</topic><topic>delayed hypersensitivity</topic><topic>Diagnosis</topic><topic>Evaluation</topic><topic>Female</topic><topic>Humans</topic><topic>Laboratory diagnosis</topic><topic>Leishmania</topic><topic>Leishmania (V.) braziliensis</topic><topic>Leishmania (V.) lainsoni</topic><topic>Leishmania - immunology</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis, Cutaneous - diagnosis</topic><topic>Leishmaniasis, Cutaneous - immunology</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Male</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Montenegro skin test</topic><topic>parasites</topic><topic>Physiological aspects</topic><topic>Skin tests</topic><topic>Skin Tests - methods</topic><topic>t-test</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Melo, Leonardo Viana</creatorcontrib><creatorcontrib>Vasconcelos Dos Santos, Thiago</creatorcontrib><creatorcontrib>Ramos, Patrícia Karla</creatorcontrib><creatorcontrib>Lima, Luciana Vieira</creatorcontrib><creatorcontrib>Campos, Marliane Batista</creatorcontrib><creatorcontrib>Silveira, Fernando Tobias</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Parasites & vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Melo, Leonardo Viana</au><au>Vasconcelos Dos Santos, Thiago</au><au>Ramos, Patrícia Karla</au><au>Lima, Luciana Vieira</au><au>Campos, Marliane Batista</au><au>Silveira, Fernando Tobias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test</atitle><jtitle>Parasites & vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2024-09-27</date><risdate>2024</risdate><volume>17</volume><issue>1</issue><spage>402</spage><epage>402</epage><pages>402-402</pages><artnum>402</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.
Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.
The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO: 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.
AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>39334233</pmid><doi>10.1186/s13071-024-06486-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Antigen-antibody reactions antigens Antigens, Protozoan - immunology Axenic amastigote antigen Axenic promastigote antigen Brazil cell-mediated immunity cutaneous leishmaniasis delayed hypersensitivity Diagnosis Evaluation Female Humans Laboratory diagnosis Leishmania Leishmania (V.) braziliensis Leishmania (V.) lainsoni Leishmania - immunology Leishmaniasis Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - immunology Leishmaniasis, Cutaneous - parasitology Male Methods Middle Aged Montenegro skin test parasites Physiological aspects Skin tests Skin Tests - methods t-test Young Adult |
| title | Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test |
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