Histomolecular profile of Helicobacter pylori strains circulating in Brazzaville (Congo)

• Published in: JGH open 2024-02, Vol.8 (2), p.e13035-n/a
• Main Authors: Atipo‐Ibara, Bienvenu H, Boumba, Anicet, Ibara Ollandzobo Ikobo, Atipo, Mongo‐Onkouo, Arnaud, Mimiesse Mounamou, Jile F, Itoua‐Ngaporo, Ngala A, Moukassa, Donatien, Atipo Ibara, Blaise I
• Format: Article
• Language: English
• Subjects: Biopsy; Brazzaville; Cag A; Cancer; Carcinogens; chronic gastritis; Endoscopy; Gastroenterology; Helicobacter pylori; Hospitals; Infections; Polymerase chain reaction; strains
• ISSN: 2397-9070; 2397-9070
• DOI: 10.1002/jgh3.13035

Background and Aim Helicobacter pylori (Hp) infection is a real public health problem in the Congo. We aimed study the histomolecular profile of Hp strains circulating in Brazzaville, Congo, in order to contribute to the improvement of Hp‐infected patients in the country. Methods This was an analytical‐transversal study carried out from January to November 2020 (i.e. a study period of 11 months) in the endoscopy centers of Brazzaville as well as the molecular biology and anatomopathology laboratories of Pointe‐Noire and Oyo. It involved 100 symptomatic patients over the age of 18 referred for upper GI endoscopy. These patients underwent gastric biopsies for histopathological analysis according to the Sydney classification and molecular analysis using the real‐time polymerase chain reaction (PCR) technique. The frequency of Hp infection was determined using real‐time PCR. PCR was also used to identify the Hp strains and assess their tropism in the gastric mucosa. Digestive symptoms, endoscopic lesions, and histopathological lesions associated with HP infection were studied. Results The incidence of Hp infection was 91%, with a female predominance of 52.75% and an average age of 46.32 years. Endoscopy revealed normal mucosa (56.14%), ulcerated lesions (12.28%), and gastritis (22.81%) in infected patients. Histopathologically, the lesions were chronic atrophic gastritis (91%), with inflammatory activity (16.46%), intestinal metaplasia (16.46%), and adenocarcinoma (3.3%). Cag A strains were present in 85.71% of cases and had no preferential tropism in the gastric mucosa. Strains carrying the Cag A gene were present in severe and serious endoscopic and histopathological lesions. Conclusion The prevalence of Hp infection is 91% in the Brazzaville population. Cag A strains circulate in high proportions and are implicated in the occurrence of severe lesions of the gastric mucosa. We carried out an analytical cross‐sectional study of Helicobacter pylori (Hp) over an 11‐month study period in 100 patients who underwent gastric biopsies for histopathological and molecular analysis. Molecular carriage of Hp was 91%, histopathological lesions were chronic atrophic gastritis (91%), with inflammatory activity (16.46%), intestinal metaplasia (16.46%), and adenocarcinoma (3.3%). Cag A strains were present in 85.71% of cases, had no preferential tropism for the gastric mucosa, and were present in severe endoscopic and histopathological lesions.