Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice

[Display omitted] •Polysaccharides extracted from Dendrobium officinale leaves could make better use of production waste.•DOLP reduces gut barrier damage and cure inflammation.•DOLP alleviated liver damage caused by drugs.•DOLP regulated gut micorbiota and metabolism and increases the abundance of p...

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Bibliographic Details
Published in:Food Chemistry: X 2022-03, Vol.13, p.100235-100235, Article 100235
Main Authors: Xie, Hualing, Fang, Jingyu, Farag, Mohamed A., Li, Zhenhao, Sun, Peilong, Shao, Ping
Format: Article
Language:English
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(s) from the Special Issue on Advances on dietary polysaccharides and oligosaccharides: structure and bioactivity by Dr. Hussain and Dr. You
Cyclophosphamide
Dendrobium officinale
Gut microbiota
Immune system
Liver protection
Polysaccharide fermentation
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Summary:[Display omitted] •Polysaccharides extracted from Dendrobium officinale leaves could make better use of production waste.•DOLP reduces gut barrier damage and cure inflammation.•DOLP alleviated liver damage caused by drugs.•DOLP regulated gut micorbiota and metabolism and increases the abundance of probiotics. In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
ISSN:2590-1575
2590-1575
DOI:10.1016/j.fochx.2022.100235