De novo transcriptome reconstruction in aquacultured early life stages of the cephalopod Octopus vulgaris

Cephalopods have been considered enigmatic animals that have attracted the attention of scientists from different areas of expertise. However, there are still many questions to elucidate the way of life of these invertebrates. The aim of this study is to construct a reference transcriptome in Octopu...

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Bibliographic Details
Published in:Scientific data 2022-10, Vol.9 (1), p.609-609, Article 609
Main Authors: Prado-Álvarez, María, Dios, Sonia, García-Fernández, Pablo, Tur, Ricardo, Hachero-Cruzado, Ismael, Domingues, Pedro, Almansa, Eduardo, Varó, Inmaculada, Gestal, Camino
Format: Article
Language:English
Subjects:
631/114/129
631/337/2019
Data Descriptor
Datasets
Developmental stages
Embryos
Hatching
Humanities and Social Sciences
multidisciplinary
Octopus vulgaris
Science
Science (multidisciplinary)
Transcriptomes
Transcriptomics
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Summary:Cephalopods have been considered enigmatic animals that have attracted the attention of scientists from different areas of expertise. However, there are still many questions to elucidate the way of life of these invertebrates. The aim of this study is to construct a reference transcriptome in Octopus vulgaris early life stages to enrich existing databases and provide a new dataset that can be reused by other researchers in the field. For that, samples from different developmental stages were combined including embryos, newly-hatched paralarvae, and paralarvae of 10, 20 and 40 days post-hatching. Additionally, different dietary and rearing conditions and pathogenic infections were tested. At least three biological replicates were analysed per condition and submitted to RNA-seq analysis. All sequencing reads from experimental conditions were combined in a single dataset to generate a reference transcriptome assembly that was functionally annotated. The number of reads aligned to this reference was counted to estimate the transcript abundance in each sample. This dataset compiled a complete reference for future transcriptomic studies in O. vulgaris . Measurement(s) Transcriptome sequencing assay Technology Type(s) RNA-seq assay (Illumina) Sample Characteristic - Organism Octopus vulgaris Sample Characteristic - Environment Ocean Sample Characteristic - Location NW Spain (Ría de Vigo, Galicia)
ISSN:2052-4463
2052-4463
DOI:10.1038/s41597-022-01735-2