Non-TZF Transcriptional Activator AtC3H12 Negatively Affects Seed Germination and Seedling Development in Arabidopsis

• Published in: International journal of molecular sciences 2022-01, Vol.23 (3), p.1572
• Main Authors: Seok, Hye-Yeon, Kim, Taehyoung, Lee, Sun-Young, Moon, Yong-Hwan
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Amino Acid Sequence; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; AtC3H12; CCCH zinc finger; Cell Nucleus - metabolism; Cloning; Drought; Gene Expression Regulation, Plant; Genes; Germination; Models, Biological; Mutation - genetics; non-TZF; Promoter Regions, Genetic - genetics; Protein Domains; Protein Transport; Proteins; Protoplasts - metabolism; Saccharomyces cerevisiae - metabolism; Seed germination; seedling development; Seedlings; Seedlings - growth & development; Seedlings - metabolism; Seeds; Seeds - growth & development; Seeds - metabolism; Subcellular Fractions - metabolism; Time Factors; Trans-Activators - chemistry; Trans-Activators - metabolism; Transcription factors; Transcriptional Activation - genetics; Transgenic plants; Zinc Fingers
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23031572

CCCH zinc finger proteins are a large protein family and are classified as either tandem CCCH zinc finger (TZF) or non-TZF proteins. The roles of TZF genes in several plants have been well determined, whereas the functions of many non-TZF genes in plants remain uncharacterized. Herein, we describe biological and molecular functions of AtC3H12, an Arabidopsis non-TZF protein containing three CCCH zinc finger motifs. AtC3H12 has orthologs in several plant species but has no paralog in Arabidopsis. -overexpressing transgenic plants (OXs) germinated slower than wild-type (WT) plants, whereas mutants germinated faster than WT plants. The fresh weight (FW) and primary root lengths of OX seedlings were lighter and shorter than those of WT seedlings, respectively. In contrast, FW and primary root lengths of seedlings were heavier and longer than those of WT seedlings, respectively. AtC3H12 was localized in the nucleus and displayed transactivation activity in both yeast and Arabidopsis. We found that the 97-197 aa region of AtC3H12 is an important part for its transactivation activity. Detection of expression levels and analysis of Arabidopsis transgenic plants harboring a :: construct showed that expression increases as the Arabidopsis seedlings develop. Taken together, our results demonstrate that AtC3H12 negatively affects seed germination and seedling development as a nuclear transcriptional activator in Arabidopsis. To our knowledge, this is the first report to show that non-TZF proteins negatively affect plant development as nuclear transcriptional activators.