Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation

We isolated isobavachalcone (IBC) from (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expression of cell cycle-...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-06, Vol.19 (6), p.1693
Main Authors: Lee, Hyejin, Li, Hua, Kweon, Minson, Choi, Youngsook, Kim, Min Jung, Ryu, Jae-Ha
Format: Article
Language:English
Subjects:
3T3-L1 adipocyte
Accumulation
Adipocytes
Adipogenesis
Angelica keiskei
Autophagy
Cell cycle
Cholesterol
clonal expansion
Danio rerio
Differentiation
Drug development
Fatty liver
High cholesterol diet
High fat diet
isobavachalcone
Liver
Obesity
Phagocytosis
Pharmacology
Proteins
Steatosis
Zebrafish
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Summary:We isolated isobavachalcone (IBC) from (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expression of cell cycle-regulating proteins. IBC also inhibited autophagic flux by inducing intracellular accumulation of LC3B and SQSTM1/p62 proteins while decreasing expression levels of regulating factors for autophagy initiation. In parallel with the inhibition of adipocyte differentiation, IBC decreased intrahepatic fat deposits and rescued the liver steatosis in high fat cholesterol diet-fed zebrafish. In this study, we found that IBC isolated from AK suppresses mitotic clonal expansion and autophagy flux of adipocytes and also shows anti-obesity activity in a high cholesterol-diet zebrafish model by decreasing intrahepatic fat deposits. These results suggest that IBC could be a leading pharmacological compound for the development of anti-obesity drugs.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19061693