Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors

The combination of genome editing and primordial germ cell (PGC) transplantation has enormous significance in the study of developmental biology and genetic breeding, despite its low efficiency due to limited number of donor PGCs. Here, we employ a combination of germplasm factors to convert blastod...

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Bibliographic Details
Published in:Nature communications 2023-12, Vol.14 (1), p.7918-7918, Article 7918
Main Authors: Wang, Xiaosi, Zhu, Junwen, Wang, Houpeng, Deng, Wenqi, Jiao, Shengbo, Wang, Yaqing, He, Mudan, Zhang, Fenghua, Liu, Tao, Hao, Yongkang, Ye, Ding, Sun, Yonghua
Format: Article
Language:English
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Aquaculture
Biology
Blastoderm
Blastomeres
Blastula
Breeding
Danio rerio
Developmental biology
Efficiency
Gametes
Genome editing
Genomes
Germ cells
Germplasm
Gobiocypris rarus
Humanities and Social Sciences
Lethality
multidisciplinary
Science
Science (multidisciplinary)
Spermatozoa
Transplantation
Zebrafish
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Summary:The combination of genome editing and primordial germ cell (PGC) transplantation has enormous significance in the study of developmental biology and genetic breeding, despite its low efficiency due to limited number of donor PGCs. Here, we employ a combination of germplasm factors to convert blastoderm cells into induced PGCs (iPGCs) in zebrafish and obtain functional gametes either through iPGC transplantation or via the single blastomere overexpression of germplasm factors. Zebrafish-derived germplasm factors convert blastula cells of Gobiocypris rarus into iPGCs, and Gobiocypris rarus spermatozoa can be produced by iPGC-transplanted zebrafish. Moreover, the combination of genome knock-in and iPGC transplantation perfectly resolves the contradiction between high knock-in efficiency and early lethality during embryonic stages and greatly improves the efficiency of genome knock-in. Together, we present an efficient method for generating PGCs in a teleost, a technique that will have a strong impact in basic research and aquaculture. Primordial germ cell (PGC) transplantation is an important technology for developmental biology and genetic breeding. Here, Wang et al. develop an approach for PGC induction that enhances the efficiency of PGC transplantation in zebrafish.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-43587-3