Equine platelet concentrate preparation and validation

• Published in: Journal of veterinary internal medicine 2019-05, Vol.33 (3), p.1500-1506
• Main Authors: Bozorgmanesh, Rana, Magdesian, K. Gary, Sutton‐Burges, Julie W., Owens, Sean D., Tablin, Fern
• Format: Article
• Language: English
• Subjects: Animals; Aspartate aminotransferase; Bicarbonates; Blood; Blood platelets; Blood Platelets - cytology; Blood Preservation - methods; Blood Preservation - veterinary; Carbon dioxide; Cell culture; Centrifugation; Centrifugation - methods; Centrifugation - veterinary; Dehydrogenases; Electrolytes; Electrolytic cells; EQUID; Glucose; Hematology - methods; Hemorrhage; horse; Horses; Horses - blood; L-Lactate dehydrogenase; Lactic acid; Male; Metabolism; Platelet Count - veterinary; Platelet-Rich Plasma - chemistry; Platelet-Rich Plasma - cytology; Potassium; Prospective Studies; Sodium; Test procedures; thrombocyte; thrombocytopenia; transfusion
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/jvim.15472

Background Development of equine platelet concentrate (PC) would aid management of cases requiring transfused platelets (PLTs), where adminstration of whole‐blood or platelet‐rich plasma (PRP) might be contraindicated. Objectives To test and validate a method for production of an equine PRP‐PC product. Animals Six healthy Thoroughbred geldings from a research herd. Methods In this prospective experimental study, whole blood was collected and processed through multiple centrifugation steps to yield 120 mL of PC. The PC was stored at 22°C and gently and continuously agitated. Measurements of PLT count, pH, and concentrations of glucose, lactate, electrolytes, lactate dehydrogenase (LDH), and aspartate aminotransferase (AST), as well as partial pressures of oxygen and carbon dioxide were performed on days 0, 1, 2, 3, 5, and 7. Platelet aggregometry and bacterial culture were also performed. Results The PC always had a PLT count of ≥550 × 103 cells/μL. Aggregometry graph amplitude (P