The diversity of Plasmodium falciparum isolates from asymptomatic and symptomatic school-age children in Kinshasa Province, Democratic Republic of Congo

Understanding Plasmodium falciparum population diversity and transmission dynamics provides information on the intensity of malaria transmission, which is needed for assessing malaria control interventions. This study aimed to determine P. falciparum allelic diversity and multiplicity of infection (...

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Bibliographic Details
Published in:Malaria journal 2023-03, Vol.22 (1), p.102-12, Article 102
Main Authors: Simpson, Shirley V, Nundu, Sabin S, Arima, Hiroaki, Kaneko, Osamu, Mita, Toshihiro, Culleton, Richard, Yamamoto, Taro
Format: Article
Language:English
Subjects:
Age
Alleles
Antigens, Protozoan - genetics
Asymptomatic
Child
Children
Democratic Republic of Congo
Democratic Republic of the Congo - epidemiology
Disease transmission
DNA
Epidemiology
Genes
Genetic diversity
Genetic Variation
Genotyping
Health facilities
Human diseases
Humans
Infections
Laboratories
Malaria
Malaria, Falciparum - epidemiology
Malaria, Falciparum - parasitology
Merozoite Surface Protein 1 - genetics
Multiplicity of infection
Nucleotide sequence
Parasitemia - parasitology
Parasites
Plasmodium falciparum
Proteins
Protozoan Proteins - genetics
Public health
School-age children
Vaccines
Vector-borne diseases
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Summary:Understanding Plasmodium falciparum population diversity and transmission dynamics provides information on the intensity of malaria transmission, which is needed for assessing malaria control interventions. This study aimed to determine P. falciparum allelic diversity and multiplicity of infection (MOI) among asymptomatic and symptomatic school-age children in Kinshasa Province, Democratic Republic of Congo (DRC). A total of 438 DNA samples (248 asymptomatic and 190 symptomatic) were characterized by nested PCR and genotyping the polymorphic regions of pfmsp1 block 2 and pfmsp2 block 3. Nine allele types were observed in pfmsp1 block2. The K1-type allele was predominant with 78% (229/293) prevalence, followed by the MAD20-type allele (52%, 152/293) and RO33-type allele (44%, 129/293). Twelve alleles were detected in pfmsp2, and the 3D7-type allele was the most frequent with 84% (256/304) prevalence, followed by the FC27-type allele (66%, 201/304). Polyclonal infections were detected in 63% (95% CI 56, 69) of the samples, and the MOI (SD) was 1.99 (0.97) in P. falciparum single-species infections. MOIs significantly increased in P. falciparum isolates from symptomatic parasite carriers compared with asymptomatic carriers (2.24 versus 1.69, adjusted b: 0.36, (95% CI 0.01, 0.72), p = 0.046) and parasitaemia > 10,000 parasites/µL compared to parasitaemia 
ISSN:1475-2875
1475-2875
DOI:10.1186/s12936-023-04528-z