Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of...

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Bibliographic Details
Published in:European journal of histochemistry 2019-05, Vol.63 (2)
Main Authors: Dall'Aglio, Cecilia, Scocco, Paola, Maranesi, Margherita, Petrucci, Linda, Acuti, Gabriele, De Felice, Elena, Mercati, Francesca
Format: Article
Language:English
Subjects:
Adipose tissue
Brief Report
Cytoplasm
Diet
Glands
immunohistochemistry
Monoclonal antibodies
Nutritional status
resistin
Sheep
Uterus
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Summary:Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of resistin in the ovine uterus. Uterine samples were collected from two groups of ewes at the end of an experimental trial during which the animals of the first group (CTRL) were fed only by grazing while those of the second one (EXP) were supplemented with barley and corn. Using a monoclonal antibody against resistin, tested by Western Blot, the immunopositive reaction was identified in the cytoplasm of epithelial lining cells and uterine glands. The endogenous production of resistin seemed to be affected by different diet, as evidenced by staining differences between the CTRL and EXP groups. Our findings support the existence of a peripheral resistin system in the sheep uterus. It is possible that this system is involved in the functionality of the uterus, which is also affected by the animal's nutritional status.
ISSN:1121-760X
2038-8306
DOI:10.4081/ejh.2019.3020