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Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis
•The PLE-SPE methodology is efficient to separate different classes of compounds.•Antimicrobial effect of black tea flavonols on pathogenic microorganisms.•Probiotic microorganismos were not impaired by tea flavonols.•Black tea flavonols resulted in a blockade of the cell cycle in G0/G1.•DNA fragmen...
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Published in: | Food chemistry advances 2022-10, Vol.1, p.100042, Article 100042 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •The PLE-SPE methodology is efficient to separate different classes of compounds.•Antimicrobial effect of black tea flavonols on pathogenic microorganisms.•Probiotic microorganismos were not impaired by tea flavonols.•Black tea flavonols resulted in a blockade of the cell cycle in G0/G1.•DNA fragmentation and altered the morphology of leukemic cells.
The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy. |
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ISSN: | 2772-753X 2772-753X |
DOI: | 10.1016/j.focha.2022.100042 |