Evaluation of the natural killer cytotoxicity and the levels of cytokines in rats with type I diabetes mellitus

Type I diabetes mellitus (insulin-dependent DM = IDDM) is a chronic disease characterized by specific destruction of pancreatic beta cells, resulting in an absolute lack of insulin. Immune mechanisms, genetic susceptibility, and environmental factors are all implicated in the pathogenesis of Type 1...

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Published in:Memórias do Instituto Oswaldo Cruz 2005-12, Vol.100 (8), p.883-887
Main Authors: Fidan, Isil, Yüksel, Sevgi, Kalkanci, Ayse, Imir, Turgut, Kustimur, Semra
Format: Article
Language:English
Subjects:
Animals
cytokines
Cytokines - blood
Cytokines - immunology
Diabetes Mellitus, Experimental - blood
Diabetes Mellitus, Experimental - etiology
Diabetes Mellitus, Experimental - immunology
Diabetes Mellitus, Type 1 - blood
Diabetes Mellitus, Type 1 - etiology
Diabetes Mellitus, Type 1 - immunology
Enzyme-Linked Immunosorbent Assay
Female
Interferon-gamma - blood
Interleukins - blood
Killer Cells, Natural - immunology
natural killer cells
PARASITOLOGY
Rats
Rats, Wistar
TROPICAL MEDICINE
Tumor Necrosis Factor-alpha - analysis
Type 1 diabetes mellitus
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Summary:Type I diabetes mellitus (insulin-dependent DM = IDDM) is a chronic disease characterized by specific destruction of pancreatic beta cells, resulting in an absolute lack of insulin. Immune mechanisms, genetic susceptibility, and environmental factors are all implicated in the pathogenesis of Type 1 diabetes. This study was aimed at determining the efficiency of cytokines, natural killer (NK) cells in the pathophysiology of IDDM. Therefore, we evaluated the plasma levels of cytokines by specific enzyme-linked immunosorbent assay (ELISA) and the cytotoxicity activity of NK cells by anti-candididal index in rats with type I diabetes. We found that the cytotoxicity activity of NK cells in IDDM groups significantly decreased compared to the control groups. The levels of interferon-gamma (IFN-gamma) in IDDM groups were slightly higher than in healthy controls. These results indicate that the changes of T H1 type cytokines such as IFN-gamma and NK cell activity can play a role in the etiology of IDDM. The data may provide new strategies for the treatment of IDDM.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762005000800010