Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology

DIP2 protein contains three members: DIP2A, DIP2B and DIP2C, and are broadly expressed in the nervous system from Drosophila to human during embryonic development. Dip2c gene-associated mutations have been reported in tumors and neuronal diseases. However, the role ofDip2cin the context of mouse emb...

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Bibliographic Details
Published in:Stem cell research 2021-05, Vol.53, p.102236-102236, Article 102236
Main Authors: Yao, Mingze, Su, Pengfei, Li, Zhengfeng, Cui, Xiaogang, Yang, Qian, Xing, Xiaoyu, Lei, Yu, Lian, Mengqiao, Ren, Tinglin, Wang, Xiedong, Zheng, Yaowu, Wu, Changxin
Format: Article
Language:English
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Summary:DIP2 protein contains three members: DIP2A, DIP2B and DIP2C, and are broadly expressed in the nervous system from Drosophila to human during embryonic development. Dip2c gene-associated mutations have been reported in tumors and neuronal diseases. However, the role ofDip2cin the context of mouse embryonic stem (mES) cells has not been explored.To investigate the biological function of Dip2c during early embryo development, we generated Dip2c-/- mES line using a CRISPR/Cas9 system. This cell line has contributed to further investigation of molecular mechanism of Dip2c during cell differentiation, as well as a cell model for screening for neurogenic drug and cancer clinical cure.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2021.102236