The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells

Cultured bovine chromaffin cells have been used extensively as a neuroendocrine model to study regulated secretion. In order to extend such experimental findings to the physiological situation, it is necessary to study mayor cellular structures affecting secretion in cultured cells with their counte...

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Bibliographic Details
Published in:Frontiers in cellular neuroscience 2017-05, Vol.11, p.135-135
Main Authors: Gimenez-Molina, Yolanda, Villanueva, José, Nanclares, Carmen, Lopez-Font, Inmaculada, Viniegra, Salvador, Francés, Maria Del Mar, Gandia, Luis, Gil, Amparo, Gutiérrez, Luis M
Format: Article
Language:English
Subjects:
Actin
Adrenal glands
Amperometry
Chromaffin cells
Chromaffin granules
confocal microscopy
Cytoskeleton
Cytosol
Experiments
F-actin cytoskeleton
Fodrin
Immunoglobulins
Labeling
Localization
Mathematical models
Microscopy
Mitochondria
Neuroscience
Organelles
Phalloidin
Physiology
Secretion
transmission electron microscopy
vesicles
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Summary:Cultured bovine chromaffin cells have been used extensively as a neuroendocrine model to study regulated secretion. In order to extend such experimental findings to the physiological situation, it is necessary to study mayor cellular structures affecting secretion in cultured cells with their counterparts present in the adrenomedullary tissue. F-actin concentrates in a peripheral ring in cultured cells, as witnessed by phalloidin-rodhamine labeling, while extends throughout the cytoplasm in native cells. This result is also confirmed when studying the localization of α-fodrin, a F-actin-associated protein. Furthermore, as a consequence of this redistribution of F-actin, we observed that chromaffin granules and mitochondria located into two different cortical and internal populations in cultured cells, whereas they are homogeneously distributed throughout the cytoplasm in the adrenomedullary tissue. Nevertheless, secretion from isolated cells and adrenal gland pieces is remarkably similar when measured by amperometry. Finally, we generate mathematical models to consider how the distribution of organelles affects the secretory kinetics of intact and cultured cells. Our results imply that we have to consider F-actin structural changes to interpret functional data obtained in cultured neuroendocrine cells.
ISSN:1662-5102
1662-5102
DOI:10.3389/fncel.2017.00135