Uncovering de novo gene birth in yeast using deep transcriptomics

• Published in: Nature communications 2021-01, Vol.12 (1), p.604-604, Article 604
• Main Authors: Blevins, William R., Ruiz-Orera, Jorge, Messeguer, Xavier, Blasco-Moreno, Bernat, Villanueva-Cañas, José Luis, Espinar, Lorena, Díez, Juana, Carey, Lucas B., Albà, M. Mar
• Format: Article
• Language: English
• Subjects: 38/91; 49/39; 631/181/735; 631/208/212/2019; 631/208/325/2484; 631/553/2716; Conserved Sequence - genetics; Deoxyribonucleic acid; DNA; Gene Expression Regulation, Fungal; Gene Regulatory Networks; Genes; Genes, Fungal; Genomes; Humanities and Social Sciences; multidisciplinary; Open reading frames; Open Reading Frames - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); Synteny; Transcription; Transcriptome - genetics; Yeast; Yeasts
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-20911-3

De novo gene origination has been recently established as an important mechanism for the formation of new genes. In organisms with a large genome, intergenic and intronic regions provide plenty of raw material for new transcriptional events to occur, but little is know about how de novo transcripts originate in more densely-packed genomes. Here, we identify 213 de novo originated transcripts in Saccharomyces cerevisiae using deep transcriptomics and genomic synteny information from multiple yeast species grown in two different conditions. We find that about half of the de novo transcripts are expressed from regions which already harbor other genes in the opposite orientation; these transcripts show similar expression changes in response to stress as their overlapping counterparts, and some appear to translate small proteins. Thus, a large fraction of de novo genes in yeast are likely to co-evolve with already existing genes. Genome-wide studies of de novo genes have tended to focus on genomic open reading frames (ORFs). Here, Blevins et al. use deep transcriptomics and synteny information to identify de novo transcripts in the yeast Saccharomyces cerevisiae , many of which are expressed from the alternative DNA strand.