The influence of Schiff base inclusion complexes with β-cyclodextrine on antibiotic production by Streptomyces hygroscopicus CH-7

• Published in: Hemijska industrija 2015, Vol.69 (1), p.9-15
• Main Authors: Ilic, Slavica, Konstantinovic, Sandra, Gojgic-Cvijovic, G., Savic, Dragisa, Veljkovic, Vlada
• Format: Article
• Language: English
• Subjects: antibiotic production; Antibiotics; Bacteria; Fermentation; Filaments; Imines; inclusion complex; Inclusion complexes; Protons; Schiff base; Streptomyces hygroscopicus; β-cyclodextrine
• ISSN: 0367-598X; 2217-7426
• DOI: 10.2298/HEMIND131113012I

A media consisting of isatin-Schiff bases and its inclusion complexes with ?-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and Azalomycine ? by Streptomyces hygroscopicus CH-7. The media with ?-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 ?g/cm3 for Hexaene H-85 and 191 ?g/cm3 for Azalomycine B. The production of Hexaene H-85 and Azalomycine is higher when ?-cyclodextrine complex is added as a nitrogen source, comparing to pure isatin-Schiff base. The maximum concentration of hexaene H-85 in medium with inclusion complex of isatin-Schiff base is 1.4-2.3 times higher than the basal medium. The maximum production of Azalomycine is 2.1-3.4 times higher in media with inclusion complex of Schiff base. During the fermentation process, the nutrient media with ?-cyclodextrine inclusion complexes with isatin-Schiff bases affect the strain morphology, since it is in the form of compact pellets, which are formed from short and long, branched filaments