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Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in...
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| Published in: | BMC research notes 2020-03, Vol.13 (1), p.135-135, Article 135 |
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| creator | Jaturapaktrarak, Chalisa Payattikul, Penpan Lohnoo, Tassanee Kumsang, Yothin Laikul, Aree Pathomsakulwong, Watcharapol Yurayart, Chompoonek Tonpitak, Walaiporn Krajaejun, Theerapong |
| description | Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species.
A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis. |
| doi_str_mv | 10.1186/s13104-020-04981-y |
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A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis.</description><identifier>ISSN: 1756-0500</identifier><identifier>EISSN: 1756-0500</identifier><identifier>DOI: 10.1186/s13104-020-04981-y</identifier><identifier>PMID: 32143691</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Antibodies ; Antibodies - blood ; Bacterial Proteins - chemistry ; Bacterial Proteins - immunology ; Care and treatment ; Case-Control Studies ; Cats ; Cattle ; Communicable diseases ; Diagnosis ; Diseases ; Dogs ; Early Diagnosis ; ELISA ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - standards ; Enzymes ; Horses ; Humans ; Identification and classification ; Immune Sera - chemistry ; Immunoassay enzymes ; Immunodiagnosis ; Immunoglobulins ; Laboratories ; Methods ; Protein A/G ; Proteins ; Pythiosis ; Pythiosis - blood ; Pythiosis - diagnosis ; Pythiosis - immunology ; Pythiosis - parasitology ; Pythium ; Pythium - immunology ; Pythium - isolation & purification ; Pythium insidiosum ; Research Note ; Sensitivity and Specificity ; Staphylococcal Protein A - chemistry ; Staphylococcal Protein A - immunology ; Tropical diseases</subject><ispartof>BMC research notes, 2020-03, Vol.13 (1), p.135-135, Article 135</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c597t-abf820f3a615d624bc87ad9bfe9772ae6bb117f2142c738c7578ab3b6c22503d3</citedby><cites>FETCH-LOGICAL-c597t-abf820f3a615d624bc87ad9bfe9772ae6bb117f2142c738c7578ab3b6c22503d3</cites><orcidid>0000-0003-0545-3765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059324/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2378980385?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32143691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jaturapaktrarak, Chalisa</creatorcontrib><creatorcontrib>Payattikul, Penpan</creatorcontrib><creatorcontrib>Lohnoo, Tassanee</creatorcontrib><creatorcontrib>Kumsang, Yothin</creatorcontrib><creatorcontrib>Laikul, Aree</creatorcontrib><creatorcontrib>Pathomsakulwong, Watcharapol</creatorcontrib><creatorcontrib>Yurayart, Chompoonek</creatorcontrib><creatorcontrib>Tonpitak, Walaiporn</creatorcontrib><creatorcontrib>Krajaejun, Theerapong</creatorcontrib><title>Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects</title><title>BMC research notes</title><addtitle>BMC Res Notes</addtitle><description>Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species.
A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>BMC research notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jaturapaktrarak, Chalisa</au><au>Payattikul, Penpan</au><au>Lohnoo, Tassanee</au><au>Kumsang, Yothin</au><au>Laikul, Aree</au><au>Pathomsakulwong, Watcharapol</au><au>Yurayart, Chompoonek</au><au>Tonpitak, Walaiporn</au><au>Krajaejun, Theerapong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects</atitle><jtitle>BMC research notes</jtitle><addtitle>BMC Res Notes</addtitle><date>2020-03-06</date><risdate>2020</risdate><volume>13</volume><issue>1</issue><spage>135</spage><epage>135</epage><pages>135-135</pages><artnum>135</artnum><issn>1756-0500</issn><eissn>1756-0500</eissn><abstract>Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species.
A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32143691</pmid><doi>10.1186/s13104-020-04981-y</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0545-3765</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Antibodies - blood Bacterial Proteins - chemistry Bacterial Proteins - immunology Care and treatment Case-Control Studies Cats Cattle Communicable diseases Diagnosis Diseases Dogs Early Diagnosis ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - standards Enzymes Horses Humans Identification and classification Immune Sera - chemistry Immunoassay enzymes Immunodiagnosis Immunoglobulins Laboratories Methods Protein A/G Proteins Pythiosis Pythiosis - blood Pythiosis - diagnosis Pythiosis - immunology Pythiosis - parasitology Pythium Pythium - immunology Pythium - isolation & purification Pythium insidiosum Research Note Sensitivity and Specificity Staphylococcal Protein A - chemistry Staphylococcal Protein A - immunology Tropical diseases |
| title | Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects |
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