Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina

Complexins (Cplxs) 1 to 4 are components of the presynaptic compartment of chemical synapses where they regulate important steps in synaptic vesicle exocytosis. In the retina, all four Cplxs are present, and while we know a lot about Cplxs 3 and 4, little is known about Cplxs 1 and 2. Here, we perfo...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (15), p.8131
Main Authors: Lux, Uwe Thorsten, Ehrenberg, Johanna, Joachimsthaler, Anneka, Atorf, Jenny, Pircher, Bianca, Reim, Kerstin, Kremers, Jan, Gießl, Andreas, Brandstätter, Johann Helmut
Format: Article
Language:English
Subjects:
Adaptation
Amacrine cells
Antibodies
Bioinformatics
complexin 1
complexin 2
conventional chemical synapses
Exocytosis
Experiments
ganglion cells
horizontal cells
Hybridization
Immunocytochemistry
Information processing
Light microscopy
Mammals
Morphology
Optical microscopy
Photoreceptors
Proteins
Retina
Retinal ganglion cells
Rodents
Signal processing
SNAP receptors
Synapses
Visual signals
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Summary:Complexins (Cplxs) 1 to 4 are components of the presynaptic compartment of chemical synapses where they regulate important steps in synaptic vesicle exocytosis. In the retina, all four Cplxs are present, and while we know a lot about Cplxs 3 and 4, little is known about Cplxs 1 and 2. Here, we performed in situ hybridization experiments and bioinformatics and exploited Cplx 1 and Cplx 2 single-knockout mice combined with immunocytochemistry and light microscopy to characterize in detail the cell type and synapse-specific distribution of Cplx 1 and Cplx 2. We found that Cplx 2 and not Cplx 1 is the main isoform expressed in normal and displaced amacrine cells and ganglion cells in mouse retinae and that amacrine cells seem to operate with a single Cplx isoform at their conventional chemical synapses. Surprising was the finding that retinal function, determined with electroretinographic recordings, was altered in Cplx 1 but not Cplx 2 single-knockout mice. In summary, the results provide an important basis for future studies on the function of Cplxs 1 and 2 in the processing of visual signals in the mammalian retina.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22158131