Effect of delayed entry of blood culture bottles in BACTEC automated blood culture system in the context of laboratory consolidation

Delayed entry of blood culture bottles is frequent in consolidated laboratories. A retrospective study evaluated time from insertion to detection and total detection time as a function of preincubation time, and we prospectively looked for false negative results. 69,604 blood culture bottles were re...

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Bibliographic Details
Published in:Scientific reports 2022-01, Vol.12 (1), p.1337-12, Article 1337
Main Authors: Deslandes, Vincent, Rafipour, Darya, Gorn, Ivan, Sabri, Elham, Sant, Nadia, Desjardins, Marc
Format: Article
Language:English
Subjects:
631/326
631/326/107
631/326/2521
Bacteriological Techniques - methods
Blood
Blood culture
Culture Media
DNA, Bacterial - analysis
Humanities and Social Sciences
Humans
Laboratories
multidisciplinary
Polymerase chain reaction
Retrospective Studies
Reviews
Science
Science (multidisciplinary)
Staphylococcus
Staphylococcus - isolation & purification
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Summary:Delayed entry of blood culture bottles is frequent in consolidated laboratories. A retrospective study evaluated time from insertion to detection and total detection time as a function of preincubation time, and we prospectively looked for false negative results. 69,604 blood culture bottles were reviewed for preincubation time, incubation time and total detection time. Positive cultures for specific bacterial subtypes were reviewed to assess the effect of preincubation time on likelihood of detection. 492 negative blood cultures were prospectively tested by 16S RNA PCR and Staphylococcus -specific PCR for the presence of bacterial DNA. Mean preincubation time for samples collected within the city-limits was 3.94 h versus 9.49–18.89 h for other client sites. Higher preincubation times were partially mitigated by a lower incubation time, with an overall increase in total detection time. A lower odds ratio of recovery of Staphylococcus spp was identified, but not confirmed by terminal subcultures and molecular assays. Prolonged preincubation of blood cultures affects total detection time despite a reduction in incubation time. Successful centralization of microbiological services may depend upon optimization of courier routes for inoculated blood culture bottles. Our data supports consideration for an increase in suggested maximum preincubation times.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-05246-3