Systematic analysis of different degrees of haemolysis on miRNA levels in serum and serum-derived extracellular vesicles from dogs

Background Circulating microRNAs (miRNAs) are described as promising non-invasive biomarkers for diagnostics and therapeutics. Human studies have shown that haemolysis occurring during blood collection or due to improper sample processing/storage significantly alters the miRNA content in plasma and...

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Published in:BMC veterinary research 2022-09, Vol.18 (1), p.355-355, Article 355
Main Authors: Aguilera-Rojas, Matias, Sharbati, Soroush, Stein, Torsten, Candela Andrade, Mario, Kohn, Barbara, Einspanier, Ralf
Format: Article
Language:English
Subjects:
Biomarker
Biomarkers
blood sampling
blood serum
diagnostic techniques
Dogs
Extracellular vesicles
Gene expression
Haemolysis
Hemolysis
humans
microRNA
MicroRNAs
miRNA
Plasma
Serum
therapeutics
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Summary:Background Circulating microRNAs (miRNAs) are described as promising non-invasive biomarkers for diagnostics and therapeutics. Human studies have shown that haemolysis occurring during blood collection or due to improper sample processing/storage significantly alters the miRNA content in plasma and serum. Nevertheless, no similar research has been performed in dogs so far. We therefore investigated the effects of different degrees of haemolysis on the levels of selected miRNAs in serum and serum-derived extracellular vesicles (EVs) from dogs, by inducing a controlled in vitro haemolysis experiment. Results The abundance of miR-16, miR-92a, miR-191, miR-451 and miR-486 was significantly sensitive to haemolysis in serum and serum-derived EVs, while other selected miRNAs were not influenced by haemolysis. Furthermore, we found that the abundance of some canine miRNAs differs from data reported in the human system. Conclusions Our results describe for the first time the impact of haemolysis on circulating miRNAs not only in whole serum, but also in serum-derived EVs from dogs. Hence, we provide novel data for further analyses in the discovery of canine circulating biomarkers. Our findings suggest that haemolysis should be carefully assessed to assure accuracy when investigating circulating miRNA in serum or plasma-based tests.
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-022-03445-8