Palladium(II) Metal Complex Fabricated Titanium Implant Mitigates Dual-Species Biofilms in Artificial Synovial Fluid

Metallodrugs have a potent application in various medical fields. In the current study, we used a novel Palladium(II) thiazolinyl picolinamide complex that was directly fabricated over the titanium implant to examine its potency in inhibiting dual-species biofilms and exopolysaccharides. Additionall...

Full description

Saved in:
Bibliographic Details
Published in:Antibiotics (Basel) 2023-08, Vol.12 (8), p.1296
Main Authors: Jothipandiyan, Sowndarya, Suresh, Devarajan, Sekaran, Saravanan, Paramasivam, Nithyanand
Format: Article
Language:English
Subjects:
Analysis
Antibiotics
Antimicrobial agents
artificial synovial fluid
Bacteria
Bacterial infections
Biocompatibility
Biofilms
Biological activity
Biological products
Biomaterials
Biomedical materials
Bone biomaterials
Coatings
Drug resistance
dual-species biofilms
Exopolysaccharides
Gene expression
Growth media
implant
Injuries
Metal plates
Methicillin
Older people
Open fractures
Orthopedics
Palladium
Palladium(II) metal complex
Pathogens
Prostheses
Scanning electron microscopy
Stability analysis
Staphylococcus infections
Surgical implants
Synovial fluid
Titanium
Transplants & implants
Trauma
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Metallodrugs have a potent application in various medical fields. In the current study, we used a novel Palladium(II) thiazolinyl picolinamide complex that was directly fabricated over the titanium implant to examine its potency in inhibiting dual-species biofilms and exopolysaccharides. Additionally, inhibition of mono- and dual-species biofilms by coated titanium plates in an in vitro joint microcosm was performed. The study was carried out for 7 days by cultivating mono- and dual-species biofilms on titanium plates placed in both growth media and artificial synovial fluid (ASF). By qPCR analysis, the interaction of co-cultured biofilms in ASF and the alteration in gene expression of co-cultured biofilms were studied. Remarkable alleviation of biofilm accumulation and EPS secretion was observed on the coated titanium plates. The effective impairment of biofilms and EPS matrix of biofilms on Pd(II)-E-coated titanium plates were visualized by Scanning Electron Microscopy. Moreover, coated titanium plates improved the adhesion of osteoblast cells, which is crucial for a bone biomaterial. The potential bioactivity of coated plates was also confirmed at the molecular level using qPCR analysis. The stability of coated plates in ASF for 7 days was examined with FESEM-EDAX analysis. Collectively, the present study provided an excellent anti-infective effect on Pd(II)-E-coated titanium plates without affecting their biocompatibility with bone cells.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics12081296