Anti-biofilm Effect of Copper Oxide Nanoparticles, EDTA, and Kojic Acid on MDR-biofilm Forming Gram Negative Superbugs in Sohag University Hospitals, Egypt

• Published in: Microbes and Infectious Diseases 2023-02, Vol.4 (1), p.108-126
• Main Authors: asmaa thabet, Amira Esmail, Mamdouh Esmat
• Format: Article
• Language: English
• Subjects: biofilm. cuonps; key words: edta; kojic acid; mdr
• ISSN: 2682-4132; 2682-4140
• DOI: 10.21608/mid.2022.163207.1404

Background: Multidrug-resistant (MDR) Gram negative superbugs are considered among the most important causes of healthcare associated infections and these resistant isolates are usually present in association with biofilms rather than planktonic existence. Objectives: This study aimed to assess the ability of EDTA, Kojic acid, and CuONPs to inhibit the phenomenon of biofilm formation by multidrug-resistant Gram-negative superbugs. Methodology: Gram negative bacilli were isolated on suitable media and identified biochemically by vitek-2 biochemical identification system. MDR isolates were tested for biofilm formation by tissue culture plate method, biofilm-producing isolates were exposed EDTA and Kojic acid by certain concentration and to copper oxide nanoparticle (CuONPs) by multiple gradual concentrations and re-tested for biofilm-production by the same method. Results: EDTA and Kojic acid reduced the biofilm-formation ability of the tested isolates by 85.4 % and 65.5% respectively with reduced mean optical density (OD) reading to (0.0267± 0.051) and (0.063± 0.108) respectively with a high statistically significant difference (p < /em>