Simultaneous Determination of Tetrodotoxin in the Fresh and Heat-Processed Aquatic Products by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Tetrodotoxin (TTX) was simultaneously detected in the fresh and heat-processed aquatic products by high-performance liquid chromatography-tandem mass spectrometry method. The detection conditions were investigated, including the chromatography column and mobile phase. Based on the optimized paramete...

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Bibliographic Details
Published in:Foods 2022-03, Vol.11 (7), p.925
Main Authors: Ye, Hongli, Xi, Yinfeng, Tian, Liangliang, Huang, Dongmei, Huang, Xuanyun, Shen, Xiaosheng, Cai, Youqiong, Wangs, Yuan
Format: Article
Language:English
Subjects:
Accuracy
Acids
Bioassays
Calibration
Chromatography
Column chromatography
Food science
Heat
heat-processed Gadus
High performance liquid chromatography
high-performance liquid chromatography–tandem mass spectrometry
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Parameter sensitivity
Scientific imaging
simultaneous determination
Spectroscopy
Statistical analysis
Takifugu
Tetrodotoxin
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Summary:Tetrodotoxin (TTX) was simultaneously detected in the fresh and heat-processed aquatic products by high-performance liquid chromatography-tandem mass spectrometry method. The detection conditions were investigated, including the chromatography column and mobile phase. Based on the optimized parameters, a sensitive determination method of TTX was established. The proposed method featured the merits of a good linear relationship between signal and TTX concentration (R = 0.9998), a wide detection matrix-based range of 0.2-100 ng/g, and a low detection limit of 0.2 ng/g, etc. The spiked assays evidenced its accuracy and reliability with recoveries of 90.5-107.2%. Finally, the developed method was simultaneously successfully applied in the determination of TTX in various fresh and heat-processed aquatic products.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods11070925