Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae

Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were id...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 1973-10, Vol.7 (5), p.319-327
Main Authors: Guerrero, Jorge, Silverman, Paul H.
Format: Article
Language:English
Subjects:
Fractionation
Larvae
TROPICAL MEDICINE
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Summary:Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs. Os antígenos metabólicos de Ascaris suum foram obtidos de larvas de segundo e de terceiro estágio (precoce) cultivadas in vitro em meio mínimo essencial de Eeagle com suplemento proteico. Os antígenos metabólicos coletados depois de 12 a 16 dias das culturas in vitro foram eluidos em Bio-Gel Al 5. Três picos de eluição foram identificados, dializados, liofilizados e injetados em camundongos, com alginato de sódio a 4%. O pico II da eluição de duas preparações dos antígenos metabólicos protegeram os camundongos da infecção com 10.000 ovos infectivos de A. suum
ISSN:0037-8682
1678-9849
0037-8682
1678-9849
DOI:10.1590/S0037-86821973000500005