Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders

The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characteriz...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2023-02, Vol.13 (4), p.699
Main Authors: Rodà, Francesca, Picciolini, Silvia, Mangolini, Valentina, Gualerzi, Alice, Seneci, Pierfausto, Renda, Antonio, Sesana, Silvia, Re, Francesca, Bedoni, Marzia
Format: Article
Language:English
Subjects:
Alzheimer's disease
Blood-brain barrier
Data analysis
Drug delivery
Drug delivery systems
Drug therapy
Drugs
Effectiveness
Glioblastoma
Inflammation
Kinases
Lipids
liposome
Liposomes
multivariate analysis
Nanoparticles
Nervous system diseases
Neurodegenerative diseases
Neurological diseases
Neurological disorders
Pharmaceutical research
Pharmaceuticals
Quality control
Raman spectra
Raman spectroscopy
Spectroscopy
Synthesis
therapeutics
Vehicles
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Summary:The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characterization of liposomes (LPs) to be tested for the control of neuroinflammation and microglial dysfunctions in Glioblastoma multiforme and Alzheimer's disease. Drug-loaded LPs were functionalized to cross the blood-brain barrier and to guarantee localized and controlled drug release. The Raman spectra of each LP component were used to evaluate their contribution in the LP Raman fingerprint. Raman data analysis made it possible to statistically discriminate LPs with different functionalization patterns, showing that each molecular component has an influence in the Raman spectrum of the final LP formulation. Moreover, CLS analysis on Raman data revealed a good level of synthetic reproducibility of the formulations and confirmed their stability within one month from their synthesis, demonstrating the ability of the technique to evaluate the efficacy of LP synthesis using small amount of sample. RS represents a valuable tool for a fast, sensitive and label free biochemical characterization of LPs that could be used for quality control of nanoparticle-based therapeutics.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano13040699