PDLIM1 inhibits cell migration and invasion in diabetic retinopathy via negatively regulating Wnt3a

• Published in: Scientific reports 2023-04, Vol.13 (1), p.5820-5820, Article 5820
• Main Authors: Xie, Pinxue, You, Qisheng, Zhu, Jiang, Xie, Wuxiang, Wei, Ping, Zhu, Siquan, Du, Yunhui, Gao, Xinxiao
• Format: Article
• Language: English
• Subjects: 631/80; 692/420; Animals; Apoptosis; Cell culture; Cell migration; Cell Movement; Diabetes; Diabetes mellitus; Diabetes Mellitus - metabolism; Diabetic retinopathy; Diabetic Retinopathy - pathology; Endothelial cells; Endothelial Cells - metabolism; Epithelium; Flow cytometry; Humanities and Social Sciences; Immunofluorescence; LIM Domain Proteins - genetics; LIM protein; Mice; multidisciplinary; Neoplastic Processes; Retina; Retina - pathology; Retinal pigment epithelium; Retinopathy; Science; Science (multidisciplinary); siRNA; Therapeutic targets; Transfection; Vascular diseases; Western blotting; Wnt protein; Wnt3A Protein - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-33073-7

The injury of vascular endothelial cells is a crucial factor in the development of diabetic retinopathy (DR). PDLIM1 (a member of the PDZ and LIM protein family) has been reported to exert an essential function in vascular diseases. This study aimed to elucidate the role of PDLIM1 on retinal vascular endothelial cells in DR. Immunofluorescence staining was used to localize the expression of PDLIM1 in the mouse retina. In some tumor diseases, PDLIM1 has been reported to play a key role in regulating the Wnt pathway. However, no in-depth reports have been found in DR. Retinal capillary endothelial cells (RCECs) were treated with high-glucose and high-lipid (HG/HL) culture medium, and siRNA transfection to investigate the role of PDLIM1 in DR. PDLIM1 and Wnt3a expression was confirmed by qRT-PCR and western blotting. Flow cytometry, Transwell assay, and scratch assay were used to test the ability of cell apoptosis, migration, and invasion. PDLIM1 was mainly expressed in the retinal pigment epithelium (RPE), ganglion cell layer (GCL), inner plexus layer (IPL), and outer plexus layer (OPL). HG/HL increased Wnt3a levels and promoted cell’s ability of apoptosis, migration, and invasion, which were reversed by the knockdown of PDLIM1. PDLIM1 was found to play a protective role in diabetic retinopathy by counter-regulating Wnt3a. PDLIM1 ameliorates cell apoptosis, migration, and invasion by negatively regulating Wnt3a in RCECs of DR, which suggests that PDLIM1 might be a promising therapeutic target for DR treatment.