IS481EU Shows a New Connection between Eukaryotic and Prokaryotic DNA Transposons

DDD/E transposase gene is the most abundant gene in nature and many DNA transposons in all three domains of life use it for their transposition. A substantial number of eukaryotic DNA transposons show similarity to prokaryotic insertion sequences (ISs). The presence of IS -like DNA transposons was i...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2023-02, Vol.12 (3), p.365
Main Authors: Kojima, Kenji K, Bao, Weidong
Format: Article
Language:English
Subjects:
Bioinformatics
Datasets
DDD/E transposase
Deoxyribonucleic acid
DNA
DNA transposon
Genomes
insertion sequence
Insertion sequences
IS481
IS481EU
Nucleotide sequence
Phylogenetics
Phylogeny
Prokaryotes
Proteins
target site duplications (TSDs)
Transposase
Transposase gene
Transposition
Transposons
Trichomonas vaginalis
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Summary:DDD/E transposase gene is the most abundant gene in nature and many DNA transposons in all three domains of life use it for their transposition. A substantial number of eukaryotic DNA transposons show similarity to prokaryotic insertion sequences (ISs). The presence of IS -like DNA transposons was indicated in the genome of . Here, we surveyed IS -like eukaryotic sequences using a bioinformatics approach and report a group of eukaryotic IS -like DNA transposons, designated , from parabasalids including . The lengths of target site duplications (TSDs) of are around 4 bps, around 15 bps, or around 25 bps, and strikingly, these discrete lengths of TSDs can be observed even in a single family. Phylogenetic analysis indicated the close relationships of with some of the prokaryotic IS family members. was not well separated from / in the phylogenetic analysis, but was distinct from other eukaryotic DNA transposons including and . The unique characteristics of in protein sequences and the distribution of TSD lengths support its placement as a new superfamily of eukaryotic DNA transposons.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology12030365