In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach

The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydroly...

Full description

Saved in:
Bibliographic Details
Published in:Antioxidants 2021-12, Vol.11 (1), p.27
Main Authors: López-Pedrouso, María, Lorenzo, José M, Borrajo, Paula, Franco, Daniel
Format: Article
Language:English
Subjects:
alcalase
amino acid profile
Amino acids
Antioxidants
Argininosuccinate synthase
Aspartic acid
ATP synthase
bromelain
Chromatography
Collagen
Dihydropyrimidinase
Enzymes
flavourzyme
Fructose-bisphosphate aldolase
Histidine
Hydrolysates
Hydrophobicity
Liver
Mass spectrometry
Meat industry
Papain
Peptides
Pork
pork by-products
Proteins
Scientific imaging
Subtilisin
Threonine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydrolysates proved to be of adequate quality regarding the ratio EAA/NEAA, particularly flavourzyme hydrolysates. The peptidomic profiles were significantly different for each enzyme and their characterizations were performed, resulting in forty-four differentially abundant peptides among the four treatments. Porcine liver hydrolysates from alcalase and bromelain are demonstrated to have the most antioxidant capacity. On the other hand, hydrophobic amino acid residues (serine, threonine, histidine and aspartic acid) might be reducing the hydrolysates antioxidant capacity. Seventeen peptides from collagen, albumin, globin domain-containing protein, cytochrome β, fructose-bisphosphate aldolase, dihydropyrimidinase, argininosuccinate synthase, and ATP synthase seem to be antioxidant. Further studies are necessary to isolate these peptides and test them in in vivo experiments.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11010027