SPARC plays an important role in the oviposition and nymphal development in Nilaparvata lugens Stål

The brown planthopper (Nilaparvata lugens Stål)is a notorious rice pest in many areas of Asia. Study on the molecular mechanisms underlying its development and reproduction will provide scientific basis for effective control. SPARC (Secreted Protein, Acidic and Rich in Cysteine) is one of structural...

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Bibliographic Details
Published in:BMC genomics 2022-10, Vol.23 (1), p.1-682, Article 682
Main Authors: Wang, Weixia, Zhu, Tingheng, Wan, Pinjun, Wei, Qi, He, Jiachun, Lai, Fengxiang, Fu, Qiang
Format: Article
Language:English
Subjects:
Abnormalities
Analysis
Control
Cysteine
Diseases and pests
Eggs
Embryonic development
Extracellular matrix
Fat body
Gene expression
Genes
Genetic aspects
Genomes
Genomics
Growth
Hemolymph
Insects
Males
Methods
Molecular modelling
Mortality
Nilaparvata lugens
Offspring
Osteonectin
Oviposition
Parental RNAi
Pesticides
Planthoppers
Prevention
Progeny
Propagation
Proteins
Reproductive organs
Rice
RNA-mediated interference
RNAi
SPARC
Survival
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Summary:The brown planthopper (Nilaparvata lugens Stål)is a notorious rice pest in many areas of Asia. Study on the molecular mechanisms underlying its development and reproduction will provide scientific basis for effective control. SPARC (Secreted Protein, Acidic and Rich in Cysteine) is one of structural component of the extracellular matrix, which influences a diverse array of biological functions. In this study, the gene for SPARC was identified and functionally analysed from N.lugens. The result showed that the NlSPARC mRNA was highly expressed in fat body, hemolymph and early embryo. The mortality increased significantly when NlSPARC was downregulated after RNA interference (RNAi) in 3 ~ 4.sup.th instar nymphs. Downregulation of NlSPARC in adults significantly reduced the number of eggs and offspring, as well as the transcription level of NlSPARC in newly hatched nymphs and survival rate in progeny. The observation with microanatomy on individuals after NlSPARC RNAi showed smaller and less abundant fat body than that in control. No obvious morphological abnormalities in the nymphal development and no differences in development of internal reproductive organ were observed when compared with control. NlSPARC is required for oviposition and nymphal development mainly through regulating the tissue of fat body in N.lugens. NlSPARC could be a new candidate target for controlling the rapid propagation of N.lugens population. Our results also demonstrated that the effect of NlSPARC RNAi can transfer to the next generation in N.lugens.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-022-08903-z