Antimicrobial efficiency and cytocompatibility of resveratrol and naringin as chemical decontaminants on SLA surface

• Published in: Microbiology spectrum 2024-10, Vol.12 (10), p.e0367923
• Main Authors: Zhou, You, Shen, Zhe, Xu, Yan, Qian, Xin-Na, Chen, Wei, Qiu, Jing
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antimicrobial efficiency; Bacterial Adhesion - drug effects; Bacteriology; Biofilms - drug effects; Cell Adhesion - drug effects; Cell Proliferation - drug effects; chemical decontaminant; cytocompatibility; Decontamination - methods; Fibroblasts - drug effects; Flavanones - chemistry; Flavanones - pharmacology; Humans; Mice; naringin; Osteoblasts - drug effects; Porphyromonas gingivalis - drug effects; Research Article; resveratrol; Resveratrol - chemistry; Resveratrol - pharmacology; Staphylococcus aureus - drug effects; Surface Properties; Titanium - chemistry; Titanium - pharmacology
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.03679-23

Bacterial biofilms are the major etiology agent of peri-implant disease. Chemical decontamination is a promising treatment strategy against bacterial biofilms; however, its applications are limited by its low efficiency and poor biocompatibility. In contrast to three conventional cleaners (sterile saline, hydrogen peroxide, and chlorhexidine), this study used resveratrol and naringin solutions to remove mature and biofilm on sandblasted (with large grit and acid-etched (SLA) titanium surface. To determine changes in surface characteristics, the surface wettability and roughness were measured, and micromorphology was observed by scanning electron microscopy. With crystal violet (CV) and live/dead bacterial staining, residual plaque quantity and composition were measured. The biocompatibility was tested using pH and cytotoxicity, as well as by osteoblasts (MC3T3-E1) adhesion, proliferation, and differentiation, and fibroblasts (L-929) proliferation were also analyzed. It was found that resveratrol and naringin solutions were more effective in restoring surface characteristics and also showed that less plaque and viable bacteria were left. Naringin removed biofilms better than chlorhexidine. Alkaline resveratrol and naringin solutions increased cell adhesion, proliferation, and osteogenic differentiation without any cytotoxicity. Resveratrol increased the expression of mRNA and protein associated with osteogenesis. In conclusion, resveratrol and naringin effectively restored SLA titanium surface characteristics and decontaminated the biofilm with good biocompatibility, suggesting their therapeutic potential as chemical decontaminants. Bacterial biofilms are considered the primary etiology of peri-implant disease. Physical cleaning is the most common way to remove bacterial biofilm, but it can cause grooving, melting, and deposition of chemicals that alter the surface of implants, which may hamper biocompatibility and re-osseointegration. Chemical decontamination is one of the most promising treatments but is limited by low efficiency and poor biocompatibility. Our study aims to develop safer, more effective chemical decontaminants for peri-implant disease prevention and treatment. We focus on resveratrol and naringin, two natural compounds, which have shown to be more effective in decontaminating biofilms on dental implant surfaces and exerting better biocompatibility. This research is groundbreaking as it is the first exploration of natural plant extracts' i