Comparative secretomic and proteomic analysis reveal multiple defensive strategies developed by Vibrio cholerae against the heavy metal (Cd2+, Ni2+, Pb2+, and Zn2+) stresses

Vibrio cholerae is a common waterborne pathogen that can cause pandemic cholera in humans. The bacterium with heavy metal-tolerant phenotypes is frequently isolated from aquatic products, however, its tolerance mechanisms remain unclear. In this study, we investigated for the first time the response...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2023-10, Vol.14, p.1294177-1294177
Main Authors: Zhang, Beiyu, Xu, Jingjing, Sun, Meng, Yu, Pan, Ma, Yuming, Xie, Lu, Chen, Lanming
Format: Article
Language:English
Subjects:
food safety
heavy metal
Microbiology
proteome
secretome
tolerance mechanism
Vibrio cholerae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vibrio cholerae is a common waterborne pathogen that can cause pandemic cholera in humans. The bacterium with heavy metal-tolerant phenotypes is frequently isolated from aquatic products, however, its tolerance mechanisms remain unclear. In this study, we investigated for the first time the response of such V. cholerae isolates ( n  = 3) toward the heavy metal (Cd 2+ , Ni 2+ , Pb 2+ , and Zn 2+ ) stresses by comparative secretomic and proteomic analyses. The results showed that sublethal concentrations of the Pb 2+ (200 μg/mL), Cd 2+ (12.5 μg/mL), and Zn 2+ (50 μg/mL) stresses for 2 h significantly decreased the bacterial cell membrane fluidity, but increased cell surface hydrophobicity and inner membrane permeability, whereas the Ni 2+ (50 μg/mL) stress increased cell membrane fluidity ( p  
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1294177