Identification of the immune subtype of ovarian cancer patients by integrated analyses of transcriptome and single-cell sequencing data

Ovarian cancer (OC) is one the most life-threatening cancers affecting women’s health worldwide. Immunotherapy has become a promising treatment for a variety of cancers, but the therapeutic effects in OC remain limited. In this study, we constructed a macrophage risk score (MRS) based on M1 and M2 m...

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Published in:Scientific reports 2022-08, Vol.12 (1), p.13296-13296, Article 13296
Main Authors: Wang, Sixue, Wang, Xi, Xia, Xiaomeng, Zhang, Tingting, Yi, Mingyu, Li, Zeying, Jiang, Li, Yang, Yalan, Fu, Jie, Fang, Xiaoling
Format: Article
Language:English
Subjects:
631/67
631/67/1517
631/67/327
CD8 antigen
Cell interactions
Humanities and Social Sciences
Immune checkpoint inhibitors
Immunotherapy
Inflammation
Lymphocytes T
Macrophages
Medical prognosis
Microenvironments
multidisciplinary
Ovarian cancer
Patients
Prognosis
Science
Science (multidisciplinary)
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Summary:Ovarian cancer (OC) is one the most life-threatening cancers affecting women’s health worldwide. Immunotherapy has become a promising treatment for a variety of cancers, but the therapeutic effects in OC remain limited. In this study, we constructed a macrophage risk score (MRS) based on M1 and M2 macrophages and a gene risk score (GRS) based on the prognostic genes associated with MRS. Next, cell–cell communication analysis was performed using single-cell RNA (scRNA) sequencing data. Survival status and immune characteristics were compared between the high- and low-score groups separated by MRS or GRS. Our results suggested that MRS and GRS can identify the immune subtypes of OC patients with better overall survival (OS) and inflammatory immune microenvironment. Moreover, M1 and M2 macrophages may affect the prognosis of OC patients through signal communication with CD8 T cells. Finally, functional differences between the two groups separated by GRS were elucidated. Taken together, this study constructed two useful models for the identification of immune subtypes in OC, which has a better prognosis and may have a sensitive response to immune checkpoint inhibitors (ICIs). The hub genes for the construction of GRS may be potential synergetic targets for immunotherapy in OC patients.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-17645-7