Visible Light Enhanced Extracellular Electron Transfer between a Hematite Photoanode and Pseudomonas aeruginosa

Exploring the interplay between sunlight, semiconducting minerals, and microorganisms in nature has attracted great attention in recent years. Here we report for the first time the investigation of the interaction between a hematite photoelectrode and Pseudomonas aeruginosa PAO1 under visible light...

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Bibliographic Details
Published in:Minerals (Basel) 2017-12, Vol.7 (12), p.230
Main Authors: Ren, Guiping, Sun, Yuan, Sun, Manyi, Li, Yan, Lu, Anhuai, Ding, Hongrui
Format: Article
Language:English
Subjects:
Analytical methods
Cell culture
Earth
Electrodes
Electron microscopy
Electron transfer
Electrons
Extracellular
extracellular electron transfer
Fluorine
Haematite
Hematite
Irradiation
Light
Light effects
Light irradiation
Microorganisms
Minerals
Oxidoreductions
photoanode
Photoanodes
Photoelectric effect
Photoelectric emission
Pseudomonas aeruginosa
Pseudomonas aeruginosa PAO1
Pyocyanin
Scanning electron microscopy
Spectroscopy
Sunlight
Tin
Tin oxide
Tin oxides
Ultraviolet radiation
Ultraviolet spectroscopy
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Summary:Exploring the interplay between sunlight, semiconducting minerals, and microorganisms in nature has attracted great attention in recent years. Here we report for the first time the investigation of the interaction between a hematite photoelectrode and Pseudomonas aeruginosa PAO1 under visible light irradiation. Hematite is the most abundant mineral on earth, with a band gap of 2.0 eV. A hematite electrode was electrochemically deposited on fluorine-doped tin oxide (FTO). It was thoroughly characterized by environmental scanning electron microscopy (ESEM), Raman, and UV-Vis spectroscopy, and its prompt response to visible light was determined by linear sweep voltammetry (LSV). Notably, under light illumination, the hematite electrode immersed in a live cell culture was able to produce 240% more photocurrent density than that in the abiotic control of the medium, suggesting a photoenhanced extracellular electron transfer process occurring between hematite and PAO1. Different temperatures of LSV measurements showed bioelectrochemical activity in the system. Furthermore, I-t curves under various conditions demonstrated that both a direct and an indirect electron transferring process occurred between the hematite photoanode and PAO1. Moreover, the indirect electron transferring route was more dominant, which may be mainly attributed to the pyocyanin biosynthesized by PAO1. Our results have expanded our understanding in that in addition to Geobacter and Shewanella it has been shown that more microorganisms are able to perform enhanced extracellular electron transfer with semiconducting minerals under sunlight in nature.
ISSN:2075-163X
2075-163X
DOI:10.3390/min7120230