Oxidative profile and protease regulator potential to predict sperm functionality in donkey (Equus asinus)

Seminal plasma (SP) of donkey stallions was evaluated using various oxidative stress parameters as well as protease and protease inhibitor activities. SP was obtained by nine donkey stallions. In addition, one donkey stallion with non-obstructive azoospermia was enrolled in this study. Free radical...

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Bibliographic Details
Published in:Scientific reports 2021-10, Vol.11 (1), p.20551-20551, Article 20551
Main Authors: Cecchini Gualandi, Stefano, Giangaspero, Brunella, Di Palma, Tommaso, Macchia, Giuseppe, Carluccio, Augusto, Boni, Raffaele
Format: Article
Language:English
Subjects:
631/136/2434/1822
631/443/494
631/61/32
Animals
Antioxidants
Azoospermia - metabolism
Azoospermia - veterinary
Equidae - metabolism
Equus asinus
Humanities and Social Sciences
Kinetics
Male
multidisciplinary
Oxidative Stress
Proteinase inhibitors
Proteolysis
Science
Science (multidisciplinary)
Semen
Semen - metabolism
Sperm
Spermatozoa - physiology
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Summary:Seminal plasma (SP) of donkey stallions was evaluated using various oxidative stress parameters as well as protease and protease inhibitor activities. SP was obtained by nine donkey stallions. In addition, one donkey stallion with non-obstructive azoospermia was enrolled in this study. Free radical scavenging activity (FRSA), the ferric reducing ability of plasma (FRAP), total antioxidant capacity (TAC), and total thiol level (TTL) were highly correlated with each other and with the protease inhibitor activity. However, only FRAP, TAC, and the nitrate/nitrite concentration (NO x ) were significantly correlated with sperm concentration, production, and kinetics. Protease inhibitor activity was highly correlated with sperm concentration and production; however, it did not correlate with sperm kinetics. The azoospermic stallion produced a lower amount of semen than the normospermic stallions and its SP showed a lower antioxidant activity when evaluated with FRAP, TAC, and TTL as well as a higher NO x and a lower protease inhibitor activity. In conclusion, the evaluation of SP oxidative profile by FRAP, TAC, and NO x may provide reliable information on donkey sperm quality whereas protease inhibitor activity may play a role as a marker of the sperm concentration in this species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-99972-9