Integrin α10β1-selected mesenchymal stem cells reduced hypercoagulopathy in a porcine model of acute respiratory distress syndrome

• Published in: Respiratory research 2023-05, Vol.24 (1), p.145-145, Article 145
• Main Authors: Edström, Dag, Niroomand, Anna, Stenlo, Martin, Uvebrant, Kristina, Bölükbas, Deniz A, Hirdman, Gabriel, Broberg, Ellen, Lim, Hooi Ching, Hyllén, Snejana, Lundgren-Åkerlund, Evy, Pierre, Leif, Olm, Franziska, Lindstedt, Sandra
• Format: Article
• Language: English
• Subjects: Acute respiratory distress syndrome; Adipose tissue; Advanced therapeutic medicinal products; Alveoli; Animal models; Animals; Basic Medicine; Body fat; Catheters; Cell and Molecular Biology; Cell- och molekylärbiologi; Clinical Medicine; Clinical trials; Cryopreservation; E coli; Good Manufacturing Practice; Health services; Immunomodulation; Injury prevention; Integrin α10β1 mesenchymal stem cells; Integrins; Klinisk medicin; Lipopolysaccharides; Lung disease; Lung Injury; Lungmedicin och allergi; Lungs; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Mortality; Ostomy; Permeability; Pulmonary arteries; Respiratory distress syndrome; Respiratory Distress Syndrome - diagnosis; Respiratory Medicine and Allergy; Retrospective Studies; Sepsis; Stem cells; Swine; Ventilators
• ISSN: 1465-993X; 1465-9921; 1465-993X; 1465-9921
• DOI: 10.1186/s12931-023-02459-6

Mesenchymal stem cells (MSCs) have been studied for their potential benefits in treating acute respiratory distress syndrome (ARDS) and have reported mild effects when trialed within human clinical trials. MSCs have been investigated in preclinical models with efficacy when administered at the time of lung injury. Human integrin α10β1-selected adipose tissue-derived MSCs (integrin α10β1-MSCs) have shown immunomodulatory and regenerative effects in various disease models. We hypothesized that integrin α10β1 selected-MSCs can be used to treat a sepsis-induced ARDS in a porcine model when administering cells after established injury rather than simultaneously. This was hypothesized to reflect a clinical picture of treatment with MSCs in human ARDS. 12 pigs were randomized to the treated or placebo-controlled group prior to the induction of mild to moderate ARDS via lipopolysaccharide administration. The treated group received 5 × 10  cells/kg integrin α10β1-selected MSCs and both groups were followed for 12 h. ARDS was confirmed with blood gases and retrospectively with histological changes. After intervention, the treated group showed decreased need for inotropic support, fewer signs of histopathological lung injury including less alveolar wall thickening and reduction of the hypercoagulative disease state. The MSC treatment was not associated with adverse events over the monitoring period. This provides new opportunities to investigate integrin α10β1-selected MSCs as a treatment for a disease which does not yet have any definitive therapeutic options.