Wisteria floribunda Agglutinin and Its Reactive-Glycan-Carrying Prostate-Specific Antigen as a Novel Diagnostic and Prognostic Marker of Prostate Cancer

• Published in: International journal of molecular sciences 2017-01, Vol.18 (2), p.261-261
• Main Authors: Hagiwara, Kazuhisa, Tobisawa, Yuki, Kaya, Takatoshi, Kaneko, Tomonori, Hatakeyama, Shingo, Mori, Kazuyuki, Hashimoto, Yasuhiro, Koie, Takuya, Suda, Yoshihiko, Ohyama, Chikara, Yoneyama, Tohru
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antigens; Area Under Curve; Benign; biomarker; Biomarkers, Tumor - blood; Biopsy; Cancer surgery; Diagnostic systems; Glycan; Glycosylation; Humans; Hyperplasia; Immunoassay; Immunohistochemistry; LacdiNAc; Male; Middle Aged; Multivariate Analysis; N-glycan; Neoplasm Grading; Neoplasm Recurrence, Local - pathology; Nomograms; Patients; Plant Lectins - metabolism; Polysaccharides; Polysaccharides - metabolism; Prognosis; Prostate - pathology; Prostate - surgery; Prostate cancer; Prostate-specific antigen; Prostate-Specific Antigen - blood; Prostate-Specific Antigen - metabolism; Prostatectomy; Prostatic Hyperplasia - blood; Prostatic Hyperplasia - pathology; Prostatic Neoplasms - blood; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - pathology; Prostatic Neoplasms - surgery; Reactivity; Receptors, N-Acetylglucosamine - metabolism; Risk Factors; ROC Curve; Tomatoes; Wisteria floribunda; Wisteria floribunda agglutinin (WFA) lectin
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18020261

agglutinin (WFA) preferably binds to LacdiNAc glycans, and its reactivity is associated with tumor progression. The aim of this study to examine whether the serum LacdiNAc carrying prostate-specific antigen-glycosylation isomer (PSA-Gi) and WFA-reactivity of tumor tissue can be applied as a diagnostic and prognostic marker of prostate cancer (PCa). Between 2007 and 2016, serum PSA-Gi levels before prostate biopsy (Pbx) were measured in 184 biopsy-proven benign prostatic hyperplasia patients and 244 PCa patients using an automated lectin-antibody immunoassay. WFA-reactivity on tumor was analyzed in 260 radical prostatectomy (RP) patients. Diagnostic and prognostic performance of serum PSA-Gi was evaluated using area under the receiver-operator characteristic curve (AUC). Prognostic performance of WFA-reactivity on tumor was evaluated via Cox proportional hazards regression analysis and nomogram. The AUC of serum PSA-Gi detecting PCa and predicting Pbx Grade Group (GG) 3 and GG ≥ 3 after RP was much higher than those of conventional PSA. Multivariate analysis showed that WFA-reactivity on prostate tumor was an independent risk factor of PSA recurrence. The nomogram was a strong model for predicting PSA-free survival provability with a -index ≥0.7. Serum PSA-Gi levels and WFA-reactivity on prostate tumor may be a novel diagnostic and pre- and post-operative prognostic biomarkers of PCa, respectively.