First Detection and Molecular Characterization of Usutu Virus in Culex pipiens Mosquitoes Collected in Romania

Usutu virus (USUV) is an emergent arbovirus in Europe causing mortality in bird populations. Similar to West Nile virus (WNV), USUV is maintained in sylvatic cycles between mosquito vectors and bird reservoirs. Spillover events may result in human neurological infection cases. Apart from indirect ev...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-03, Vol.11 (3), p.684
Main Authors: Prioteasa, Florian Liviu, Dinu, Sorin, Tiron, Georgiana Victorița, Stancu, Ioana Georgeta, Fălcuță, Elena, Ceianu, Cornelia Svetlana, Cotar, Ani Ioana
Format: Article
Language:English
Subjects:
Aquatic insects
BG-Sentinel trap
Bird populations
Birds
CDC gravid trap
CDC light trap
Communication
Culex pipiens
Culex pipiens mosquitoes
Disease control
Metropolitan areas
Mosquitoes
phylogenetic analysis
Phylogenetics
Phylogeny
Proteins
Serology
Usutu virus
Vector-borne diseases
Vectors
Viruses
West Nile virus
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Summary:Usutu virus (USUV) is an emergent arbovirus in Europe causing mortality in bird populations. Similar to West Nile virus (WNV), USUV is maintained in sylvatic cycles between mosquito vectors and bird reservoirs. Spillover events may result in human neurological infection cases. Apart from indirect evidence provided by a recent serological study in wild birds, the circulation of USUV in Romania was not assessed. We aimed to detect and molecular characterize USUV circulating in mosquito vectors collected in South-Eastern Romania-a well-known WNV endemic region-during four transmission seasons. Mosquitoes were collected from Bucharest metropolitan area and Danube Delta, pooled, and screened by real-time RT-PCR for USUV. Partial genomic sequences were obtained and used for phylogeny. USUV was detected in s.l. female mosquitoes collected in Bucharest, in 2019. The virus belonged to Europe 2 lineage, sub-lineage EU2-A. Phylogenetic analysis revealed high similarity with isolates infecting mosquito vectors, birds, and humans in Europe starting with 2009, all sharing common origin in Northern Italy. To our knowledge, this is the first study characterizing a strain of USUV circulating in Romania.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11030684