Phytosterols Supplementation Reduces Endothelin-1 Plasma Concentration in Moderately Hypercholesterolemic Individuals Independently of Their Cholesterol-Lowering Properties

• Published in: Nutrients 2020-05, Vol.12 (5), p.1507
• Main Authors: Oliveira Godoy Ilha, Angela, Sutti Nunes, Valeria, Silva Afonso, Milessa, Regina Nakandakare, Edna, da Silva Ferreira, Guilherme, de Paula Assis Bombo, Renata, Rodrigues Giorgi, Ricardo, Marcondes Machado, Roberta, Carlos Rocha Quintão, Eder, Lottenberg, Ana Maria
• Format: Article
• Language: English
• Subjects: Adult; Aged; Apolipoproteins B; Arteriosclerosis; Atherosclerosis; Biomarkers; Biomarkers - blood; Body mass index; Brazil; C-Reactive Protein; Cardiovascular diseases; Cell adhesion & migration; Cholesterol; Cholesterol - blood; Cholesterol, LDL - blood; Clinical trials; Cross-Over Studies; Diet; Dietary Supplements; Double-Blind Method; Endothelin 1; Endothelin-1 - blood; Endothelins; Female; Fibrinogen; Gene expression; High density lipoprotein; Humans; Hypercholesterolemia - blood; Hypercholesterolemia - drug therapy; Inflammation; Interleukin 6; Lipids; Lipids - blood; Low density lipoprotein; Male; Middle Aged; Phytosterols; Phytosterols - pharmacology; Plant-based beverages; Plasma; Plasma levels; Soy Milk - administration & dosage; Soy products; Soya bean milk; Sterols; Sterols - blood; Triglycerides; Triglycerides - blood; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vascular cell adhesion molecule 1
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12051507

Experimental and clinical studies have demonstrated the effect of phytosterols (PS) on reducing plasma levels of cholesterol and LDL-c, but the effects of plant sterols beyond cholesterol-lowering are still questionable. Since inflammation and endothelial dysfunction are involved in the pathogenesis of atherosclerosis, this study aims to evaluate the effect of PS on biomarkers involved in atherosclerosis progression and whether these effects are independent of alterations in plasma LDL-c levels. Thirty-eight moderately hypercholesterolemic volunteers (58 ± 12 years; LDL-c ≥ 130 mg/dL) were randomly assigned to consume 400 mL/day of soy milk or soy milk + PS (1.6 g/day) for 4 weeks in a double-blind, placebo-controlled, cross-over study. Blood samples were collected and lipid profiles and biomarkers for inflammation and endothelial dysfunction determined. The results showed that PS treatment reduced endothelin-1 plasma concentration by 11% ( = 0.02) independently of variations in plasma levels of LDL-c. No alterations were observed regarding fibrinogen, IL-6, hs-CRP, SAA, TNFα, or VCAM-1 between placebo and PS-treated groups. Furthermore, PS reduced total plasma cholesterol concentration (-5,5%, < 0.001), LDL-c (-6.4%, < 0.05), triglycerides (-8.3%, < 0.05), and apo B (-5.3%, < 0.05), without changing HDL-c concentration ( > 0.05). Therefore, PS supplementation effectively lowers endothelin-1 independently of the reductions in plasma levels of LDL-c, contributing to the comprehension of the effect of plant sterols on endothelial function and prevention of cardiovascular diseases.