Chemical composition, genotoxicity and antigenotoxicity study of Artemisia herba-alba using the eye and wing SMART assay of Drosophila melanogaster

• Published in: Arabian journal of chemistry 2021-03, Vol.14 (3), p.102976, Article 102976
• Main Authors: Amkiss, Sanae, Dalouh, Abdelkrim, Idaomar, Mohamed
• Format: Article
• Language: English
• Subjects: Antigenotoxicity; Artemisia herba-alba; GS-MS; MMS; SMART Assays
• ISSN: 1878-5352; 1878-5379
• DOI: 10.1016/j.arabjc.2020.102976

The purpose of this study was to determine the chemical composition and to evaluate the genotoxic and antigentoxic activites of the Moroccan Artemisia herba-alba ethanolic extract. The ethanolic extract of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy (GC-MS). The mutagenic and antimutagenic activities were evaluated using thesomatic mutation and recombination test (SMART) on Drosophila melanogaster. Methyl methanesulfonate (MMS) was used as the positive control. Twenty-one compounds of the Artemisia herba-alba ethanol extract were identified; the main monoterpenes identified are camphor, α-thujone and β-thujone. Ethanolic extract of Artemisia herba-alba did not show gentotoxicity at doses used in SMART Assays. In the co-treatment, a dose-dependent decrease in mutation frequency was observed for this plant. Ethanolic extract of Artemisia herba-alba at 1.5% demonstrated a marked decrease in MMS with a strong inhibitory effect (80%). The results demonstrate that the Artemisia herba-alba exerted a significant and potent anti-mutagenic activity in wing and eye-spot of D. melanogaster.