Loading…
The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans
Candida albicans is a leading cause of fungal infections in immunocompromised patients. Management of candidemia relies on a few antifungal agents, with fluconazole being first line therapy. The emergence of fluconazole-resistant strains highlights the pressing need to improve our molecular understa...
Saved in:
| Published in: | Scientific reports 2024-11, Vol.14 (1), p.29012-10, Article 29012 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c460t-84bfbd70acdb98197c97e6c0a2ef08d1fdda9b3fa8331eddc258ab78e5079fd33 |
| container_end_page | 10 |
| container_issue | 1 |
| container_start_page | 29012 |
| container_title | Scientific reports |
| container_volume | 14 |
| creator | Stevens, Irene Silao, Fitz Gerald Huch, Susanne Liu, Honglian Ryman, Kicki Carvajal-Jimenez, Adriana Ljungdahl, Per O. Pelechano, Vicent |
| description | Candida albicans
is a leading cause of fungal infections in immunocompromised patients. Management of candidemia relies on a few antifungal agents, with fluconazole being first line therapy. The emergence of fluconazole-resistant strains highlights the pressing need to improve our molecular understanding of the drug response mechanisms. By sequencing the 5’P mRNA degradation intermediates, we establish that co-translational mRNA decay occurs in
C. albicans
and characterize how in vivo 5´-3´ exonuclease degradation trails the last translating ribosome. Thus, the study of the 5’ Phosphorylated mRNA degradome (5PSeq) offers a simple and affordable way to measure ribosome dynamics and identify codon specific ribosome stalls in response to drugs and amino acid deprivation. Building upon this, we combine RNA-Seq and 5PSeq to study the early response of sensitive and resistant
C. albicans
isolates to fluconazole. Our results highlight that transcriptional responses, rather than changes in ribosome dynamics, are the main driver of
Candida
resistance to fluconazole. |
| doi_str_mv | 10.1038/s41598-024-80435-w |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_a416378a8c954ddcb8bccd8e9940aa4b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_a416378a8c954ddcb8bccd8e9940aa4b</doaj_id><sourcerecordid>3132143381</sourcerecordid><originalsourceid>FETCH-LOGICAL-c460t-84bfbd70acdb98197c97e6c0a2ef08d1fdda9b3fa8331eddc258ab78e5079fd33</originalsourceid><addsrcrecordid>eNp9kk1v1DAQhiMEolXpH-CAInHhEvBXEvuE0IqPSpW4lLM1sSdbL1k72ElX5dfj3Syl2wO-2J555vXYfoviNSXvKeHyQxK0VrIiTFSSCF5Xu2fFOSOirhhn7Pmj9VlxmdKG5FEzJah6WZxxVbeyoe15sbu5xRIhDvflFMEnE904ueBhKMHbcgxpqp4mIqYx-ISpnELZD7PJ0d9hwNL5MqFPbnJ3eCjPpEsT-Klc5a2zUMLQOZPlXhUvehgSXh7ni-LHl883q2_V9fevV6tP15URDZkqKbq-sy0BYzslqWqNarExBBj2RFraWwuq4z1Izilaa1gtoWsl1qRVveX8orhadG2AjR6j20K81wGcPgRCXGuIkzMDahC04a0EaVQtslQnO2OsRKUEARBd1qoWrbTDce5O1I6hn3mFWipO2zbzHxc-Z7ZoDfr8ksNJ2WnGu1u9Dnea0lo2st53_-6oEMOvGdOkty4ZHAbwGOakOeWMCs4lzejbJ-gmzDF_10IRRhuxp9hCmRhSitg_dEOJ3vtKL77S2Vf64Cu9y0VvHt_joeSvizLAjw-TU36N8d_Z_5H9A_rv3mI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3132021641</pqid></control><display><type>article</type><title>The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans</title><source>PubMed (Medline)</source><source>Publicly Available Content Database</source><source>Full-Text Journals in Chemistry (Open access)</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Stevens, Irene ; Silao, Fitz Gerald ; Huch, Susanne ; Liu, Honglian ; Ryman, Kicki ; Carvajal-Jimenez, Adriana ; Ljungdahl, Per O. ; Pelechano, Vicent</creator><creatorcontrib>Stevens, Irene ; Silao, Fitz Gerald ; Huch, Susanne ; Liu, Honglian ; Ryman, Kicki ; Carvajal-Jimenez, Adriana ; Ljungdahl, Per O. ; Pelechano, Vicent</creatorcontrib><description>Candida albicans
is a leading cause of fungal infections in immunocompromised patients. Management of candidemia relies on a few antifungal agents, with fluconazole being first line therapy. The emergence of fluconazole-resistant strains highlights the pressing need to improve our molecular understanding of the drug response mechanisms. By sequencing the 5’P mRNA degradation intermediates, we establish that co-translational mRNA decay occurs in
C. albicans
and characterize how in vivo 5´-3´ exonuclease degradation trails the last translating ribosome. Thus, the study of the 5’ Phosphorylated mRNA degradome (5PSeq) offers a simple and affordable way to measure ribosome dynamics and identify codon specific ribosome stalls in response to drugs and amino acid deprivation. Building upon this, we combine RNA-Seq and 5PSeq to study the early response of sensitive and resistant
C. albicans
isolates to fluconazole. Our results highlight that transcriptional responses, rather than changes in ribosome dynamics, are the main driver of
Candida
resistance to fluconazole.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-024-80435-w</identifier><identifier>PMID: 39578617</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/514/1949 ; 631/326/193/2541 ; 631/326/421 ; 631/337/1645/2020 ; 631/337/2019 ; Amino acids ; Antifungal agents ; Antifungal Agents - pharmacology ; Candida albicans ; Candida albicans - drug effects ; Candida albicans - genetics ; Candidemia ; Drug Resistance, Fungal - genetics ; Exonuclease ; Fluconazole ; Fluconazole - pharmacology ; Gene Expression Regulation, Fungal - drug effects ; Humanities and Social Sciences ; Humans ; Immunocompromised hosts ; Intermediates ; Microbial Sensitivity Tests ; mRNA turnover ; multidisciplinary ; Post-transcription ; Ribonucleic acid ; Ribosomes - drug effects ; Ribosomes - metabolism ; RNA ; RNA Stability - drug effects ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Science ; Science (multidisciplinary) ; Transcription, Genetic - drug effects</subject><ispartof>Scientific reports, 2024-11, Vol.14 (1), p.29012-10, Article 29012</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c460t-84bfbd70acdb98197c97e6c0a2ef08d1fdda9b3fa8331eddc258ab78e5079fd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3132021641/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3132021641?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39578617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:160297429$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Stevens, Irene</creatorcontrib><creatorcontrib>Silao, Fitz Gerald</creatorcontrib><creatorcontrib>Huch, Susanne</creatorcontrib><creatorcontrib>Liu, Honglian</creatorcontrib><creatorcontrib>Ryman, Kicki</creatorcontrib><creatorcontrib>Carvajal-Jimenez, Adriana</creatorcontrib><creatorcontrib>Ljungdahl, Per O.</creatorcontrib><creatorcontrib>Pelechano, Vicent</creatorcontrib><title>The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Candida albicans
is a leading cause of fungal infections in immunocompromised patients. Management of candidemia relies on a few antifungal agents, with fluconazole being first line therapy. The emergence of fluconazole-resistant strains highlights the pressing need to improve our molecular understanding of the drug response mechanisms. By sequencing the 5’P mRNA degradation intermediates, we establish that co-translational mRNA decay occurs in
C. albicans
and characterize how in vivo 5´-3´ exonuclease degradation trails the last translating ribosome. Thus, the study of the 5’ Phosphorylated mRNA degradome (5PSeq) offers a simple and affordable way to measure ribosome dynamics and identify codon specific ribosome stalls in response to drugs and amino acid deprivation. Building upon this, we combine RNA-Seq and 5PSeq to study the early response of sensitive and resistant
C. albicans
isolates to fluconazole. Our results highlight that transcriptional responses, rather than changes in ribosome dynamics, are the main driver of
Candida
resistance to fluconazole.</description><subject>631/1647/514/1949</subject><subject>631/326/193/2541</subject><subject>631/326/421</subject><subject>631/337/1645/2020</subject><subject>631/337/2019</subject><subject>Amino acids</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - pharmacology</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - genetics</subject><subject>Candidemia</subject><subject>Drug Resistance, Fungal - genetics</subject><subject>Exonuclease</subject><subject>Fluconazole</subject><subject>Fluconazole - pharmacology</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunocompromised hosts</subject><subject>Intermediates</subject><subject>Microbial Sensitivity Tests</subject><subject>mRNA turnover</subject><subject>multidisciplinary</subject><subject>Post-transcription</subject><subject>Ribonucleic acid</subject><subject>Ribosomes - drug effects</subject><subject>Ribosomes - metabolism</subject><subject>RNA</subject><subject>RNA Stability - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transcription, Genetic - drug effects</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk1v1DAQhiMEolXpH-CAInHhEvBXEvuE0IqPSpW4lLM1sSdbL1k72ElX5dfj3Syl2wO-2J555vXYfoviNSXvKeHyQxK0VrIiTFSSCF5Xu2fFOSOirhhn7Pmj9VlxmdKG5FEzJah6WZxxVbeyoe15sbu5xRIhDvflFMEnE904ueBhKMHbcgxpqp4mIqYx-ISpnELZD7PJ0d9hwNL5MqFPbnJ3eCjPpEsT-Klc5a2zUMLQOZPlXhUvehgSXh7ni-LHl883q2_V9fevV6tP15URDZkqKbq-sy0BYzslqWqNarExBBj2RFraWwuq4z1Izilaa1gtoWsl1qRVveX8orhadG2AjR6j20K81wGcPgRCXGuIkzMDahC04a0EaVQtslQnO2OsRKUEARBd1qoWrbTDce5O1I6hn3mFWipO2zbzHxc-Z7ZoDfr8ksNJ2WnGu1u9Dnea0lo2st53_-6oEMOvGdOkty4ZHAbwGOakOeWMCs4lzejbJ-gmzDF_10IRRhuxp9hCmRhSitg_dEOJ3vtKL77S2Vf64Cu9y0VvHt_joeSvizLAjw-TU36N8d_Z_5H9A_rv3mI</recordid><startdate>20241122</startdate><enddate>20241122</enddate><creator>Stevens, Irene</creator><creator>Silao, Fitz Gerald</creator><creator>Huch, Susanne</creator><creator>Liu, Honglian</creator><creator>Ryman, Kicki</creator><creator>Carvajal-Jimenez, Adriana</creator><creator>Ljungdahl, Per O.</creator><creator>Pelechano, Vicent</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope><scope>DOA</scope></search><sort><creationdate>20241122</creationdate><title>The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans</title><author>Stevens, Irene ; Silao, Fitz Gerald ; Huch, Susanne ; Liu, Honglian ; Ryman, Kicki ; Carvajal-Jimenez, Adriana ; Ljungdahl, Per O. ; Pelechano, Vicent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-84bfbd70acdb98197c97e6c0a2ef08d1fdda9b3fa8331eddc258ab78e5079fd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>631/1647/514/1949</topic><topic>631/326/193/2541</topic><topic>631/326/421</topic><topic>631/337/1645/2020</topic><topic>631/337/2019</topic><topic>Amino acids</topic><topic>Antifungal agents</topic><topic>Antifungal Agents - pharmacology</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Candida albicans - genetics</topic><topic>Candidemia</topic><topic>Drug Resistance, Fungal - genetics</topic><topic>Exonuclease</topic><topic>Fluconazole</topic><topic>Fluconazole - pharmacology</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunocompromised hosts</topic><topic>Intermediates</topic><topic>Microbial Sensitivity Tests</topic><topic>mRNA turnover</topic><topic>multidisciplinary</topic><topic>Post-transcription</topic><topic>Ribonucleic acid</topic><topic>Ribosomes - drug effects</topic><topic>Ribosomes - metabolism</topic><topic>RNA</topic><topic>RNA Stability - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Transcription, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stevens, Irene</creatorcontrib><creatorcontrib>Silao, Fitz Gerald</creatorcontrib><creatorcontrib>Huch, Susanne</creatorcontrib><creatorcontrib>Liu, Honglian</creatorcontrib><creatorcontrib>Ryman, Kicki</creatorcontrib><creatorcontrib>Carvajal-Jimenez, Adriana</creatorcontrib><creatorcontrib>Ljungdahl, Per O.</creatorcontrib><creatorcontrib>Pelechano, Vicent</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevens, Irene</au><au>Silao, Fitz Gerald</au><au>Huch, Susanne</au><au>Liu, Honglian</au><au>Ryman, Kicki</au><au>Carvajal-Jimenez, Adriana</au><au>Ljungdahl, Per O.</au><au>Pelechano, Vicent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2024-11-22</date><risdate>2024</risdate><volume>14</volume><issue>1</issue><spage>29012</spage><epage>10</epage><pages>29012-10</pages><artnum>29012</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Candida albicans
is a leading cause of fungal infections in immunocompromised patients. Management of candidemia relies on a few antifungal agents, with fluconazole being first line therapy. The emergence of fluconazole-resistant strains highlights the pressing need to improve our molecular understanding of the drug response mechanisms. By sequencing the 5’P mRNA degradation intermediates, we establish that co-translational mRNA decay occurs in
C. albicans
and characterize how in vivo 5´-3´ exonuclease degradation trails the last translating ribosome. Thus, the study of the 5’ Phosphorylated mRNA degradome (5PSeq) offers a simple and affordable way to measure ribosome dynamics and identify codon specific ribosome stalls in response to drugs and amino acid deprivation. Building upon this, we combine RNA-Seq and 5PSeq to study the early response of sensitive and resistant
C. albicans
isolates to fluconazole. Our results highlight that transcriptional responses, rather than changes in ribosome dynamics, are the main driver of
Candida
resistance to fluconazole.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>39578617</pmid><doi>10.1038/s41598-024-80435-w</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2024-11, Vol.14 (1), p.29012-10, Article 29012 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_a416378a8c954ddcb8bccd8e9940aa4b |
| source | PubMed (Medline); Publicly Available Content Database; Full-Text Journals in Chemistry (Open access); Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 631/1647/514/1949 631/326/193/2541 631/326/421 631/337/1645/2020 631/337/2019 Amino acids Antifungal agents Antifungal Agents - pharmacology Candida albicans Candida albicans - drug effects Candida albicans - genetics Candidemia Drug Resistance, Fungal - genetics Exonuclease Fluconazole Fluconazole - pharmacology Gene Expression Regulation, Fungal - drug effects Humanities and Social Sciences Humans Immunocompromised hosts Intermediates Microbial Sensitivity Tests mRNA turnover multidisciplinary Post-transcription Ribonucleic acid Ribosomes - drug effects Ribosomes - metabolism RNA RNA Stability - drug effects RNA, Messenger - genetics RNA, Messenger - metabolism Science Science (multidisciplinary) Transcription, Genetic - drug effects |
| title | The early transcriptional and post-transcriptional responses to fluconazole in sensitive and resistant Candida albicans |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T16%3A06%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20early%20transcriptional%20and%20post-transcriptional%20responses%20to%20fluconazole%20in%20sensitive%20and%20resistant%20Candida%20albicans&rft.jtitle=Scientific%20reports&rft.au=Stevens,%20Irene&rft.date=2024-11-22&rft.volume=14&rft.issue=1&rft.spage=29012&rft.epage=10&rft.pages=29012-10&rft.artnum=29012&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-024-80435-w&rft_dat=%3Cproquest_doaj_%3E3132143381%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c460t-84bfbd70acdb98197c97e6c0a2ef08d1fdda9b3fa8331eddc258ab78e5079fd33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3132021641&rft_id=info:pmid/39578617&rfr_iscdi=true |