Evaluation of the effect of IL-22 on human cord blood CD4+ T cells

IL-22 is a member of IL-10 cytokine family which is believed to play an important role in inflammatory responses. IL-22 has similarities with IL-10 including conserved sequences with IL-10. IL-22 receptor is also comprised of two chains known as L-22R1 and L-10R2; supporting the speculation that the...

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Published in:Iranian journal of allergy, asthma, and immunology asthma, and immunology, 2010-06, Vol.9 (2), p.59-67
Main Authors: Arasteh, Javad, Pourpak, Zahra, Ebtekar, Masoumeh, Pourfathollah, Ali Akbar, Hassan, Zuhair Mohammad, Farahmandian, Tahereh, Niknam, Hamid Mahmoudzadeh
Format: Article
Language:English
Subjects:
CD2 Antigens - blood
CD28 Antigens - blood
CD3 Complex - blood
CD4-Positive T-Lymphocytes - drug effects
Cells, Cultured
Cord blood
Fetal Blood - cytology
Forkhead Transcription Factors - blood
Foxp3
Humans
IL-22
Interleukin-22
Interleukins - pharmacology
Lymphocyte Activation - drug effects
Regulatory T cells
T-Lymphocytes, Regulatory - drug effects
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Summary:IL-22 is a member of IL-10 cytokine family which is believed to play an important role in inflammatory responses. IL-22 has similarities with IL-10 including conserved sequences with IL-10. IL-22 receptor is also comprised of two chains known as L-22R1 and L-10R2; supporting the speculation that the two cytokines may have similar effects. The aim of this study was to shed some light on the biological activity of IL-22 upon the cord blood CD4+CD25- T cells. In this research, cord blood T CD4+CD25- cells were cultured in presence of anti CD2/CD3/CD28 coated beads, IL-2 and IL-22 for two weeks at 37 degrees C and 5% CO2. Flow cytometry analysis showed that IL-22 has no effect upon CD25 and Foxp3 expression. Also, the results indicated that IL-22 is not involved in CD4+ T cell proliferation. Moreover, the results of suppression assay did not show any suppression effect on the cultured T cells. Thus, it seems that umbilical cord blood T cells probably do not express IL-22R1 on their surface.
ISSN:1735-1502
1735-5249