Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife

Wolbachia endosymbionts are a proven target for control of human disease caused by filarial nematodes. However, little is known about the occurrence of Wolbachia in taxa closely related to the superfamily Filarioidea. Our study addressed the status of Wolbachia presence in members of the superfamily...

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Published in:Parasites & vectors 2014-03, Vol.7 (1), p.140-140
Main Authors: Foster, Jeremy M, Landmann, Frédéric, Ford, Louise, Johnston, Kelly L, Elsasser, Sarah C, Schulte-Hostedde, Albrecht I, Taylor, Mark J, Slatko, Barton E
Format: Article
Language:English
Subjects:
Animals
Animals, Wild
antiserum
Brugia malayi
cytochrome-c oxidase
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Dracunculiasis - epidemiology
Dracunculiasis - parasitology
Dracunculiasis - veterinary
Dracunculoidea
Dracunculus
Dracunculus medinensis
Dracunculus Nematode - microbiology
endosymbionts
Female
Filarioidea
fluorescence
Genetic testing
Ghana - epidemiology
Guinea worm
human diseases
Humans
immunohistochemistry
Infections
Mink - parasitology
Nematoda
Ontario - epidemiology
Otters - parasitology
Parasites
polymerase chain reaction
ribosomal RNA
screening
Short Report
Species Specificity
Spirurida
surface proteins
Wildlife
Wolbachia
Wolbachia - isolation & purification
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cited_by cdi_FETCH-LOGICAL-b685t-a5e57b8fa6d262514103597e6d637cbea20ceac99040693af72177c5e936c8003
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container_title Parasites & vectors
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creator Foster, Jeremy M
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Slatko, Barton E
description Wolbachia endosymbionts are a proven target for control of human disease caused by filarial nematodes. However, little is known about the occurrence of Wolbachia in taxa closely related to the superfamily Filarioidea. Our study addressed the status of Wolbachia presence in members of the superfamily Dracunculoidea by screening the human parasite Dracunculus medinensis and related species from wildlife for Wolbachia. D. medinensis, D. lutrae and D. insignis specimens were all negative for Wolbachia colonization by PCR screening for the Wolbachia ftsZ, 16S rRNA and Wolbachia surface protein (wsp) sequences. The quality and purity of the DNA preparations was confirmed by amplification of nematode 18S rRNA and cytochrome c oxidase subunit I sequences. Furthermore, Wolbachia endobacteria were not detected by whole mount fluorescence staining, or by immunohistochemistry using a Wolbachia-specific antiserum. In contrast, positive control Brugia malayi worms were shown to harbour Wolbachia by PCR, fluorescence staining and immunohistochemistry. Three examined species of Dracunculus showed no evidence of Wolbachia endobacteria. This supports that members of the superfamily Dracunculoidea are free of Wolbachia. Within the order Spirurida, these endosymbionts appear restricted to the Filarioidea.
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subjects Animals
Animals, Wild
antiserum
Brugia malayi
cytochrome-c oxidase
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Dracunculiasis - epidemiology
Dracunculiasis - parasitology
Dracunculiasis - veterinary
Dracunculoidea
Dracunculus
Dracunculus medinensis
Dracunculus Nematode - microbiology
endosymbionts
Female
Filarioidea
fluorescence
Genetic testing
Ghana - epidemiology
Guinea worm
human diseases
Humans
immunohistochemistry
Infections
Mink - parasitology
Nematoda
Ontario - epidemiology
Otters - parasitology
Parasites
polymerase chain reaction
ribosomal RNA
screening
Short Report
Species Specificity
Spirurida
surface proteins
Wildlife
Wolbachia
Wolbachia - isolation & purification
title Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
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