Fingerprinting of Plum ( Prunus domestica ) Genotypes in Lithuania Using SSR Markers

This study's aim was to evaluate the genetic diversity of European plum ( ) cultivars and hybrids in Lithuania using SSR markers. In total, 107 plum genotypes (including 68 European plum cultivars and 39 hybrids) from the genetic resources collection of the Institute of Horticulture of the Lith...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2023-04, Vol.12 (7), p.1538
Main Authors: Antanynienė, Raminta, Šikšnianienė, Jūratė Bronė, Stanys, Vidmantas, Frercks, Birutė
Format: Article
Language:English
Subjects:
Agricultural research
Alleles
Analysis
Bayesian analysis
Biological diversity
Cluster analysis
Cultivars
Fingerprinting
Genetic analysis
Genetic aspects
Genetic diversity
Genetic markers
Genetic research
Genetic resources
Genomes
Genotype
Genotype & phenotype
Genotypes
Germplasm
Horticulture
Hybrids
Microsatellites
Microsatellites (Genetics)
Molecular biology
Physiological aspects
Plant genetics
Plant resources
Plum
Plums
Prunus domestica
Research facilities
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study's aim was to evaluate the genetic diversity of European plum ( ) cultivars and hybrids in Lithuania using SSR markers. In total, 107 plum genotypes (including 68 European plum cultivars and 39 hybrids) from the genetic resources collection of the Institute of Horticulture of the Lithuanian Research Centre for Agriculture and Forestry (LRCAF IH) were evaluated using nine microsatellite markers (SSRs) previously published and suggested by the European Cooperative Programme for Plant Genetic Resources (ECPGR). Up to six alleles per locus with each primer pair were generated for some genotypes due to the hexaploidy of plums. The number of alleles in each primer ranged from 18 to 30, with an average of 24.33. The highest number of alleles was generated with the PacA33 primer pair (30). The most informative primer, according to the PIC value, was BPPCT007. Sixty-two unique alleles (representing 39.5% of all polymorphic alleles) have been detected in the plum germplasm developed in Lithuania. According to UPGMA cluster analysis, 58 European plum genotypes were separated into eight groups without any relation to fruit color or shape. By genetic diversity (UPGMA) and structure (Bayesian) analysis, European plum hybrids were grouped into clusters according to their pedigree.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12071538