Enhancement of tendon-bone interface healing and graft maturation with cylindrical titanium-web (TW) in a miniature swine anterior cruciate ligament reconstruction model: histological and collagen-based analysis

Tendon-bone interface healing and ligamentization of the graft in anterior cruciate ligament (ACL) reconstruction with autografts are important factors affecting treatment outcome. This study aimed to investigate the effectiveness of a cylindrical titanium-web (TW) in tendon-bone interface healing a...

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Bibliographic Details
Published in:BMC musculoskeletal disorders 2020-03, Vol.21 (1), p.198-198, Article 198
Main Authors: Ryu, Keisho, Saito, Mitsuru, Kurosaka, Daisaburo, Kitasato, Seiichiro, Omori, Toshiyuki, Hayashi, Hiroteru, Kayama, Tomohiro, Marumo, Keishi
Format: Article
Language:English
Subjects:
ACL reconstruction
Analysis
Animals
Anterior cruciate ligament
Anterior Cruciate Ligament - pathology
Anterior Cruciate Ligament - surgery
Anterior Cruciate Ligament Reconstruction - methods
Autografts
Autografts - surgery
Biocompatibility
Bone grafts
Bone healing
Collagen
Collagen - metabolism
Collagen maturation
Cross-linking
Disease Models, Animal
Enthesis
Ethanol
Female
Growth factors
Knee
Ligaments
Limbs
Microscopy
Miniature swine
Musculoskeletal diseases
Orthopedic surgery
Patellar Ligament - transplantation
Surgery
Surgical Mesh
Swine
Swine, Miniature
Tendon-bone interface
Tendons
Tibia
Tibia - pathology
Tibia - physiopathology
Titanium
Titanium - chemistry
Titanium-web
Transplantation, Autologous
Transplantation, Homologous
Wound Healing
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Summary:Tendon-bone interface healing and ligamentization of the graft in anterior cruciate ligament (ACL) reconstruction with autografts are important factors affecting treatment outcome. This study aimed to investigate the effectiveness of a cylindrical titanium-web (TW) in tendon-bone interface healing and graft maturation in ACL reconstruction. Fourteen mature female CLAWN miniature swine underwent bilateral ACL reconstructions with patellar tendon (PT) autografts. In one limb, the TW/tendon complex was placed into the proximal side of the tibial tunnel. Only the graft was transplanted into the tunnel in the control limb. The proximal side of the graft was sutured into the stump of the native ACL and the distal end was stapled to the tibia. The animals were euthanized at 4 and 15 weeks postoperatively, for histological and biochemical analyses. Microscopic images in TW limbs showed that ingrowth of tendon-like tissue and mineralized bone tissue into the TW connected the bone and the tendon directly. In contrast, fibrous tissue intervened between the bone and tendon in the control limbs. The total amount of collagen cross-links (which defines the strength of collagen fibers) and the maturation of collagen cross-links in TW tendons were significantly higher (p 
ISSN:1471-2474
1471-2474
DOI:10.1186/s12891-020-03199-0