Interstitial Arabidopsis -Type Telomeric Repeats in Asteraceae

Tandem repeats of telomeric-like motifs at intra-chromosomal regions, known as interstitial telomeric repeats (ITR), have drawn attention as potential markers of structural changes, which might convey information about evolutionary relationships if preserved through time. Building on our previous wo...

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Bibliographic Details
Published in:Plants (Basel) 2021-12, Vol.10 (12), p.2794
Main Authors: Maravilla, Alexis J, Rosato, Marcela, Álvarez, Inés, Nieto Feliner, Gonzalo, Rosselló, Josep A
Format: Article
Language:English
Subjects:
Anacyclus
Arabidopsis
Arabidopsis-type ITR
Asteraceae
Asterales
Chromosomes
Conveying
cytogenetic evolution
Cytogenetics
FISH
Fluorescence
Fluorescence in situ hybridization
interstitial telomeric repeats
Karyotypes
Phylogenetics
Phylogeny
Taxonomy
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Summary:Tandem repeats of telomeric-like motifs at intra-chromosomal regions, known as interstitial telomeric repeats (ITR), have drawn attention as potential markers of structural changes, which might convey information about evolutionary relationships if preserved through time. Building on our previous work that reported outstanding ITR polymorphisms in the genus , we undertook a survey across 132 Asteraceae species, focusing on the six most speciose subfamilies and considering all the ITR data published to date. The goal was to assess whether the presence, site number, and chromosomal location of ITRs convey any phylogenetic signal. We conducted fluorescent in situ hybridization (FISH) using an -type telomeric sequence as a probe on karyotypes obtained from mitotic chromosomes. FISH signals of ITR sites were detected in species of subfamilies Asteroideae, Carduoideae, Cichorioideae, Gymnarhenoideae, and Mutisioideae, but not in Barnadesioideae. Although six small subfamilies have not yet been sampled, altogether, our results suggest that the dynamics of ITR formation in Asteraceae cannot accurately trace the complex karyological evolution that occurred since the early diversification of this family. Thus, ITRs do not convey a reliable signal at deep or shallow phylogenetic levels and cannot help to delimitate taxonomic categories, a conclusion that might also hold for other important families such as Fabaceae.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10122794