EGCG Regulates Cell Apoptosis of Human Umbilical Vein Endothelial Cells Grown on 316L Stainless Steel for Stent Implantation

• Published in: Drug design, development and therapy development and therapy, 2021-01, Vol.15, p.493-499
• Main Authors: Wang, Jinpeng, Wang, Yue, Zhao, Yuyi, Zhao, Jinbin, Zhang, Beilin, Xu, Kun
• Format: Article
• Language: English
• Subjects: 316 stainless steel; Apoptosis; Apoptosis - drug effects; Atherosclerosis; Austenitic stainless steels; Autophagy; Biotechnology industry; Cardiovascular disease; Caspase-3; Catechin - analogs & derivatives; Catechin - pharmacology; Cell adhesion & migration; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Drug delivery; Drug development; Drugs; egcg; Endothelial cells; Epigallocatechin-3-gallate; Flow cytometry; Gene expression; Human Umbilical Vein Endothelial Cells - drug effects; Humans; huvecs; Implantation; Implants; Molecular Structure; Morphology; Original Research; Phagocytosis; Polymer coatings; Polymers; Restenosis; Software; Stainless steel; Stainless Steel - pharmacology; Statistical analysis; Stents; Structure-Activity Relationship; Surgical implants; Thromboembolism; Thrombosis; Umbilical vein; Variance analysis
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/DDDT.S296548

Nowadays, medical grade 316L stainless steel (316L SS) is being widely used for intravascular stents, and the drug-eluting stent (DES) system is able to significantly reduce the occurrences of in-stent restenosis. But the drugs and the polymer coating used in DES potentially induce the forming of late stent thrombosis. In order to reduce the occurrence of ISR after stent implantation, the development of novel drugs for DESs is urgently needed. This study aimed to investigate the potential mechanisms of epigallocatechin-3-gallate (EGCG) on human umbilical vein endothelial cells (HUVEC) grown on 316L stainless steel (316L SS) using flow cytometry and Q-PCR methods. Our results showed that EGCG (12.5, 25, 50, 100 μmol/L) significantly inhibited HUVEC proliferation. Flow cytometry analysis indicated that EGCG (25, 50, 100 μmol/L) induced apoptosis. Moreover, qRT-PCRrevealed that genes associated with cell apoptosis (caspase-3, 8, 9, Fas) and autophagy (Atg 5, Atg 7, Atg 12) were up-regulated after EGCG treatment. These findings indicate that EGCG possesses chemo preventive potential in stent coating which may serve as a novel new drug for stent implantation.