Biomarker identification of isolated compartments of the cell wall, cytoplasm and vacuole from the internodal cell of characean Nitellopsis obtusa

Cells of characean algae are attractive for plant cell physiologists because of their large size and their close relation to higher plant cells. The objective of our study was to evaluate the purity of the compartments (cell wall, cytoplasm with plastids, mitochondria, nuclei and endomembrane system...

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Published in:PeerJ (San Francisco, CA) CA), 2021-02, Vol.9, p.e10930-e10930, Article e10930
Main Authors: Gylytė, Brigita, Jurkonienė, Sigita, Cimmperman, Reda, Šveikauskas, Vaidevutis, Manusadžianas, Levonas
Format: Article
Language:English
Subjects:
Algae
Analysis
Biochemistry
Biomarkers
Cell Biology
Cell compartments
Cell wall
Cell walls
Cells
Chloroplasts
Contamination
Cytoplasm
Dehydrogenases
Enzymes
Fractionation
Freshwater Biology
Localization
Malate dehydrogenase
Mannosidase
Metabolites
Mitochondria
Nanoparticles
Nitellopsis obtusa
Plant cells
Plant Science
Plastids
Proteins
Vacuole
Vacuoles
Xenobiotics
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Summary:Cells of characean algae are attractive for plant cell physiologists because of their large size and their close relation to higher plant cells. The objective of our study was to evaluate the purity of the compartments (cell wall, cytoplasm with plastids, mitochondria, nuclei and endomembrane system, and vacuole) separated mechanically from the internodal cells of using enzymatic markers. These included -mannosidase and malate dehydrogenase, vacuolar and cytoplasmic enzymes, respectively. The biomarkers applied revealed the degree of compartment contamination with the material from unwanted cell parts. The cell wall was contaminated slightly by vacuole and cytoplasm residuals, respectively by 12.3 and 1.96% of corresponding biomarker activities. Relatively high activity of vacuolar marker in the cell wall could be associated with the cell vacuoles in the multicellular structure of the nodes. The biomarkers confirmed highly purified vacuolar (99.5%) and cytoplasmic (86.7%) compartments. Purity estimation of the cell fractions enabled reevaluating nCuO related Cu concentrations in the compartments of charophyte cell. The internalisation of CuO nanoparticles in cell occurred already after 0.5h. In general, the approach seems to be useful for assessing the accumulation and distribution of various xenobiotics and/or metabolites within plant cell. All this justifies internodal cells as a model organism for modern studies in cell biology and nanotoxicology.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.10930